| BA606 |
spe-6(hc49) unc-25(e156) III; eDp6 (III;f). |
Animals with the Duplication are WT. Animals without the Duplication are Unc and Sterile. |
| BW1535 |
dpy-18(e364) ctDf3 unc-25(e156)/qC1 [dpy-19(e1259) glp-1(q339)] III. |
Heterozygotes are WT and segregate WT, DpySteriles and dead eggs. Maintain by picking WT. |
| BW1561 |
dpy-18(e364) nob-1(ct223) unc-25(e156) III; eDp6 (III;f). |
Animals with the duplication are WT. Animals without the duplication are Nob (NO Back end; 100% lethal). Pick wild-type to maintain. The Dpy and Unc phenotypes are not visible in the Nob background. ct223 is recessive. |
| CB156 |
unc-25(e156) III. |
Shrinker-contracts both dorsally and ventrally when prodded. Slow, poor backing. Slightly small. |
| CZ1632 |
juIs76 II; max-1(ju39) V. |
juIs76 [unc-25p::GFP + lin-15(+)] II. Very mild Unc. |
| CZ1758 |
juIs76 II; max-1(ju142) V. |
juIs76 [unc-25p::GFP + lin-15(+)] II. Very mild Unc. |
| CZ18412 |
juSi94 II; rps-18(ok3353) IV; glo-4(ok623) V; juEx5515. |
juSi94 [GFP11::rps-18 + Cbr-unc-119(+)] II. juEx5515 [unc-25p::GFP1-10 + unc-25p::mCherry::rab-3 + ttx-3p::RFP]. Pick ttx-3::RFP to maintain. GABAergic motor neuron-specific expression of split GFP reporter allows visualization of ribosomes in neurons, and GABAergic motor neuron-specific expression of mCherry::rab-3. Reference: Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 10.7554/eLife.26376. |
| CZ1931 |
juIs76 II; unc-71(ju156) III. |
juIs76 [unc-25p::GFP + lin-15(+)] II. Unc. |
| CZ1935 |
juIs76 II; unc-71(ju160) III. |
juIs76 [unc-25p::GFP + lin-15(+)] II. Unc. |
| CZ333 |
juIs1 IV. |
juIs1 [unc-25p::snb-1::GFP + lin-15(+)] IV. GFP expression in presynaptic terminals of GABAergic DD and VD motor neurons and RME neurons. Maintain under normal conditions. Reference: Hallan SJ and Jin Y. Nature. 1998 Sep 3;395(6697):78-82. |
| DR104 |
dpy-18(e364) unc-25(e156) dnj-17(ju1162) III. |
DpyUnc. |
| IC700 |
juIs73 II; vab-2(ju1) IV. |
juIs73 [unc-25p::GFP] II. |
| JJ532 |
pie-1(zu154) unc-25(e156)/qC1 [dpy-19(e1259) glp-1(q339)] III. |
Heterozygotes are WT and segregate WT, Unc and DpySteriles. Uncs give only dead eggs. Maintain by picking WT. |
| JT94 |
unc-25(sa94) III. |
Temperature sensitive. Weak allele. |
| ML456 |
ale-1(mc14)/spe-6(hc49) unc-25(e156) III. |
Heterozygotes are WT and segregate WT, embryonic lethals (embryos fail to elongate) and Sterile Uncs. mc14 identified as a mutation that leads to abnormal lin-26 expression. |
| MT6184 |
unc-52(e444) II; unc-25(e156) dnj-17(ju1162) III; dpy-4(e1166) IV. |
|
| MT6214 |
unc-25(n2379) III. |
Con, Shk(ts). Weak allele of unc-25. |
| MT6490 |
unc-25(n2569) III. |
Temperature sensitive. At 25C, constitutive shrinker. At 20C, non-shrinker constitutive weak. |
| OH14551 |
unc-25(ot867[unc-25::SL2::1xNLS::GFP::H2B]) III. |
unc-25(ot867[unc-25::SL2::1xNLS::GFP::H2B]) III. |
| OH4121 |
juIs76 II; wrk-1(ok695) X. |
juIs76 [unc-25p::GFP + lin-15(+)] II. |
| OH4128 |
juIs76 II; evIs82b IV. |
juIs76 [unc-25p::GFP + lin-15(+)] II. evIs82b [unc-129::GFP + dpy-20(+)] IV. |
| RM2711 |
unc-25(e156) III; snf-11(ok156) V. |
Superficially similar to unc-25 mutants (Shrinker, Exp, etc.), except that unc-25-dependent behaviors are not rescued by exogenous GABA. Reference: Mullen GP, et al. Mol Biol Cell. 2006 Jul;17(7):3021-30. |
| RM2715 |
snf-3(ok293) II; unc-25(e156) III. |
Superficially similar to unc-25 mutants (Shrinker, Exp, etc.). GABA-dependent phenotypes are rescued by exogenous GABA. Reference: Mullen GP, et al. Mol Biol Cell. 2006 Jul;17(7):3021-30. Peden AS, et al. Nat Neurosci. 2013 Dec;16(12):1794-801. |
| RM2717 |
snf-3(ok293) II; unc-25(e156) III; snf-11(ok156) V. |
Superficially the same as unc-25 mutants (Shrinker, Exp, etc.). Exogenous GABA does not rescue the unc-25-dependent expulsion deficit. See Mullen GP, et al. [Mol Biol Cell. 2006 Jul;17(7):3021-30.] for detailed description of snf-11 and unc-25; snf-11 phenotypes. See Peden AS, et al. [Nat Neurosci. 2013 Dec;16(12):1794-801]. for detailed description of snf-3 phenotypes. |
| RP1 |
trIs10. |
trIs10 [myo-3p::MB::YFP + myo-2p::YFP + ceh-23::HcRed + unc-25::DsRed + unc-129nsp::CFP]. |
| SP1104 |
unc-25(e156) bli-5(e518) III. |
Bli. Unc. |
| VC1433 |
unc-25(ok1901) III. |
Y37D8A.23. Superficially wild type. External left primer: GCTTCAACATTCCAACCGAT. External right primer: TTTGCCACCGAACTCTCTTT. Internal left primer: GGCTCAACTGTCTACGGAGC. Internal right primer: TTTTGAGAAGGGGAGGAAGG. Internal WT amplicon: 3008 bp. Estimated deletion size: 1700. Breakpoints only narrowed due to poor sequence quality. Deletion of approximately 1700 bp lies between chromosome III coordinates 12948249 and 12950465. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 |
| ZM54 |
hpIs3 X. |
hpIs3 [unc-25p::syd-2::GFP] X. GFP is expressed in small puncta along ventral and dorsal cords; bright perinuclear staining in DD and VD cell bodies. hpIs3/+ and hpIs3 males have reduced GFP levels in cell bodies. Reference: Yeh E, et al. J Neurosci. 2005 Apr 13;25(15):3833-41. |
| ZM588 |
fsn-1(hp1) III; juIs1 IV; scd-2(ok565) V. |
juIs1 [unc-25p::snb-1::GFP + lin-15(+)] IV. Animals are WT looking. In WT, GABAergic synapses visualized with juIs1 (GABAergic nervous system specific synaptobrevin::GFP) marker show uniformly spaced and sized puncta. fsn-1(hp1); scd-2(ok565) animals have puncta close to WT shape. This is more evident in larvae than in adults. |