| BA585 |
spe-10(hc104) unc-76(e911) V. |
Unc. Temperature sensitive, maintain at 16C. Average progeny: at 16C= 7 +/- 5; at 25C= 0.1 +/- .1. |
| BC4586 |
unc-76(e911) rol-9(sc148)/sC4(s2172) [dpy-21(e428)] V. |
Heterozygotes are WT and segregate WT and Unc Rollers. sC4(s2172) is not viable as a homozygote. As a heterozygote it reduces recombination between unc-76 and rol-9 to 1.8%.
Note: This strain has been sequenced and the sC4 balancer contains a large deletion from V:16,060,619 to V:19,331,432 that removes 1,279 genes and has a complex rearrangement on LGIV. See Maroilley et al. Sci Reports (2021)11:18258 for more details.
doi.org/10.1038/s41598-021-97764-9 |
| BS518 |
ozDf1/sdc-3(y52y180) unc-76(e911) V. |
Heterozygotes are slow growing with WT phenotype. Hets segregate more slow growing WT, embryonic lethals (ozDf1/ozDf1) and DpyUncs which are sick and have a maternal effect lethal (none of the offspring from the DpyUncs survive to reproduce). Maintain by picking WT. |
| CB2065 |
dpy-11(e224) unc-76(e911) V. |
DpyUnc. |
| CU6102 |
skr-1(sm151) I; unc-76(e911) V. |
sm151 is a semi-dominant allele of skr-1. Maintain under normal conditions. Reference: Killian DJ, et al. (2008) Dev Biol. 322(2):322-31. |
| CU7905 |
smIs350 IV; unc-76(e911) V. |
smIs350 [hsp-16::mCherry-NLS + tra-2::FLAG(3x) + unc-76(+)] IV. Some sterility. Maintain under normal conditions. Reference: Mapes J, et al. (2010) PNAS In press. |
| CU9087 |
unc-76(e911) V; smIs380. |
smIs380 [tra-2::GFP + unc-76(+)]. Some sterility. Maintain under normal conditions. Reference: Mapes J, et al. (2010) PNAS In press. |
| DA709 |
+/nT1 IV; sqt-3(sc63) eat-6(ad467) unc-76(e911)/nT1 V. |
Heterozygotes are WT and segregate WT, Vul and Sqt Unc larvae that grow very slowly. |
| DA869 |
sqt-3(sc8) lin-25(n545) him-5(e1467) unc-76(e911) V. |
Roller. Unc. Vulvaless. Throws males. sc8 previously called rol-4(sc8). |
| DR188 |
daf-11(m47) unc-76(e911) V. |
Temperature sensitive dauer constitutive. Dauer recovery poor at 15C. Unc. |
| DR96 |
unc-76(e911) V. |
Coiler Unc. Recessive. Easily scored. |
| EL129 |
ego-3(om40) unc-76(e911) V/nT1 [unc-?(n754) let-?] (IV;V). |
Heterozygotes are Unc and segregate additional hets, Unc-76 om40 homozygotes and dead eggs. om40 animals have multiple germline defects. |
| HS1215 |
unc-76(e911) V; osEx211. |
osEx211[apr-1::GFP + unc-76(+)]. This strain expresses functional APR-1::GFP driven by the apr-1 promoter. In the seam cells, just prior to the onset of mitosis, APR-1::GFP localizes to the anterior cortex. |
| HS1257 |
unc-76(e911) V; osEx219. |
osEx219 [pbrm-1::GFP + unc-76(+)]. Pick wild-type to maintain. GFP expression in most somatic nuclei. Reference: Shibata Y, et al. Dev Biol. 2012 Jan 15;361(2):349-57. |
| HS1294 |
unc-76(e911) V; osEx225. |
osEx225 [scm::dsh-2::venus + unc-76(+)]. Pick non-Unc to maintain. Reference: Mizumoto K, Sawa H. Dev Cell. 2007 Feb;12(2):287-99. |
| HS1325 |
unc-76(e911) V; osEx229. |
osEx229 [pry-1::GFP + unc-76(+)]. This strain expresses functional pry-1::GFP driven by the pry-1 promoter. In the seam cells, just prior to the onset of mitosis, pry-1::GFP localizes to the anterior cortex. |
| HS1359 |
unc-76(e911) V; osEx233. |
osEx233 [scm::mig-5::venus + unc-76(+)]. Pick non-Unc to maintain. Reference: Mizumoto K, Sawa H. Dev Cell. 2007 Feb;12(2):287-99. |
| HS1380 |
unc-76(e911) V; osEx240. |
osEx240 [bet-1p::bet-1::GFP + unc-76(+)]. Pick Wild-type (non-Unc) to maintain. GFP expression in most somatic cells. Reference: Shibata Y, et al. Development. 2010 Apr;137(7):1045-53. |
| HS2329 |
unc-76(e911) V; osEx397. |
osEx397 [cwn-1p::cwn-1::Venus + unc-76(+)]. Pick nonUnc to maintain. Transgene is expressed in tail region. Reference: Yamamoto et al. PLoS Genet. 2011 Oct;7(10):e1002308. |
| HS2332 |
unc-76(e911) V; osEx393. |
osEx393 [cwn-2p::cwn-2::Venus + unc-76(+)]. Pick nonUnc to maintain. Transgene is expressed in the pharynx. Reference: Yamamoto et al. PLoS Genet. 2011 Oct;7(10):e1002308. |
| HS321 |
him-5(e1467) unc-76(e911) V; osEx67. |
osEx67 [psa-4::GFP + unc-76(+)]. Maintain by picking non-Unc. Reference: Sawa H, et al. Mol Cell. 2000 Sep;6(3):617-24. |
| HS325 |
him-5(e1467) unc-76(e911) V; osEx71. |
osEx71 [psa-1::GFP + unc-76(+)]. Maintain by picking non-Unc. Reference: Sawa H, et al. Mol Cell. 2000 Sep;6(3):617-24. |
| HS942 |
unc-76(e911) V; osEx158. |
osEx158 [wrm-1::GFP + unc-76(+)]. Animals with the array are WT and GFP+. Animals which have lost the array are Unc and GFP-. |
| IA123 |
cdc-25.1(ij48) I; unc-76(e911) ijIs10 V. |
ijIs10 [cpr-5::GFP::lacZ + unc-76(+)]. Extra intestinal cells. |
| JR113 |
sma-1(e30) unc-76(e911) wDf2/sqt-3(sc8) unc-61(e228) V. |
Heterozygotes are WT and segregate WT, RolUncs and dead eggs. Homozygous wDf1 embryos arrest uniformly as unenclosed balls of differentiated cells. wDf2 formerly called zen-1(w1). sc8 previously called rol-4(sc8). |
| JR41 |
unc-76(e911) wDf1/unc-61(e228) dpy-21(e428) V. |
Heterozygotes are WT and segregate WT, DpyUncs and dead eggs. Homozygous wDf1 embryos arrest uniformly as unenclosed balls of differentiated cells. wDf1 formerly called zen-1(e2482). 2/02: dpy-21 appears to have been lost from this strain. |
| ML743 |
rdy-2(mc40)/sqt-3(sc63) him-5(e1467) unc-76(e911) V. |
Heterozygotes are WT and segregate WT, Rol Uncs, and dead L2 larvae that are translucent and often found away from the bacterial lawn (can be difficult to spot on the lawn). |
| MT10408 |
lin-53(n833) I; unc-76(e911) V; lin-15A(n767) X; nEx998. |
nEx998 [lin-53::GFP + unc-76(+)]. Pick non-Unc, non-Muv to maintain. |
| MT10865 |
unc-76(e911) V; nEx1039. |
nEx1039 contains [ced-10p::GFP::ced-10 + unc-76(+)]. Maintain at 20 C. Described in Lunquist et al., Development 128, 4475-4488 (2001). |
| MT2663 |
sqt-3(sc63) him-5(e1467) egl-1(n986) unc-76(e911) V. |
Dominant Egl. Unc. Dpy (ts). Throws males. |
| MT3553 |
egl-43(n997) II; unc-76(e911) V. |
n997: Egl, 5HT-S, IMIP-R. |
| MT5439 |
sqt-3(sc8) unc-76(e911) V; lon-2(e678) xol-1(y70) X. |
Roller. Long. Unc. XO Lethal. sc8 previously called rol-4(sc8). |
| MT6160 |
unc-76(n2397) V. |
Unc. Null allele of unc-76. |
| MT6161 |
unc-76(n2398) V. |
Unc. Weak allele of unc-76. |
| MT6292 |
unc-76(n2457) V. |
Unc. |
| MT6940 |
dpy-20(e1282) IV; unc-76(e911) V. |
Unc. Temperature sensitive Dpy. |
| MT9926 |
efl-1(n3318)/unc-76(e911) dpy-21(e428) V. |
Heterozygotes are WT and segregate WT, UncDpy and Mel. Received new stock from the Horvitz lab 5/04. |
| NJ388 |
unc-76(rh116) V. |
Unc. Loss-of-function or null allele. |
| NW424 |
unc-76(ev424) V. |
Unc. Loss-of-function or null allele. |
| OC235 |
sun-1(bs12) unc-76(e911) V. |
Unc. bs12 mutation causes sublethal defect in attachment of centrosome to the nucleus in early embryos. Viable 15-25 C. Reference: Kemp et al. (2007) Genetics 176:95-113. |
| OH8421 |
dpy-11 lsy-20(ot219) unc-76 V. |
Whole genome sequenced strain. |
| SP1493 |
sma-1(e30) unc-76(e911)/vab-8(e1017) V. |
Heterozygotes are WT and segregate WT, SmaUnc and animals with a posterior half that is thin, pale, uncoordinated. |
| SS268 |
dpy-11(e224) mes-4(bn23) unc-76(e911) V/nT1 [unc-?(n754) let-?] (IV;V). |
Heterozygotes are Unc (n754 is a dominant Unc and recessive lethal). Throws DpyUncs which give sterile progeny. The maternal effect sterility is 99% expressed, 100% strict, and is associated with 2% maternal effect embryonic lethality. |
| TY1077 |
C25D7.12(y128) unc-76(e911)/sdc-3(y52) unc-76(e911) V; xol-1(y9) X. |
Heterozygotes are Unc and segregate Uncs, Dpy Uncs [C25D7.12(y128) unc-76(e911) homozygotes], and Tra Uncs [sdc-3(y52) unc-76(e911) homozygotes]. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| TY1388 |
C25D7.12(y128)/sdc-3(y52) unc-76(e911) V. |
Heterozygotes are WT and segregate WT, Dpys [C25D7.12(y128) homozygotes], and Tra Uncs [sdc-3(y52) unc-76(e911) homozygotes]. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| TY832 |
yDf4/dpy-11(e224) unc-76(e911) V. |
Heterozygotes are WT and segregate WT, DpyUnc and dead eggs. Maintain by picking WT. |
| TY873 |
yDf6/unc-76(e911) V. |
Heterozygotes are WT and segregate WT, Unc, and dead eggs. Maintain by picking WT. |
| TY903 |
yDf7/unc-76(e911) V. |
Heterozygotes are WT and segregate WT, Unc and dead eggs. Maintain by picking WT. |
| TY956 |
sdc-3(y132)/unc-76(e911) V. |
Heterozygotes are WT and segregate WT, Unc and Dpy (sdc-3/sdc-3). sdc-3 homozygotes exhibit a strong maternal effect lethality->most progeny from homozygotes arrest as L1 larvae--about 14% escape the lethality and develop into Dpy, Egl hermaphrodites. |
| VT581 |
dpy-5(e61) lin-28(n719) I; lin-46(ma164) unc-76(e911) V. |
Dpy Unc. Egl+. lin-46 suppresses precocious Egl- phenotype of lin-28. lin-46 alone makes gaps in adult alae; enhanced at 15C. |