Gene Information: him-3

Namehim-3 View on WormBase
Species C. elegans
SequenceZK381.1
Genetic positionIV:3.27 +/- 0.002 cM
Genomic positionIV: 6977508..6978765

Strains carrying this gene

Strain Genotype Description
GE2929 unc-32(e189) pna-2(t1434)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs.
GE2935 let-725(t1440) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. t1440 previously called mel-27.
GE2940 unc-32(e189) pnm-2(t1445)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs.
GE2941 unc-32(e189) let-(t1446)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Heterozygotes are WT and segregate WT, DpySteriles and Uncs which give only dead eggs. This strain was mistakenly called emb-30 in the paper; it is not an emb-30 allele.
GE2946 let-748(t1452) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs.
GE2948 unc-32(e189) apo-2(t1454)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs.
GE2952 unc-32(e189) pnm-3(t1458)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs.
GE2959 unc-32(e189) tbg-1(t1465)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. tbg-1(t1465) previously called sas-3(t1465).
GE2961 unc-32(e189) csg-4(t1467)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs.
GE3023 emb-8(t1533) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs.
GE3633 unc-32(e189) cyk-4(t1689)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs.
JH2874 pgl-1(ct131) him-3(e1147) III; ozIs5. ozIs5 [gld-1::GFP + unc-119(+)].
JH2877 fbf-2(q738) II; pgl-1(ct131) him-3(e1147) III; axIs1722. axIs1722 [pie-1p::GFP::H2B::fog-1 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. Unknown if ed3 is still in background.
JH2881 pgl-1(ct131) him-3(e1147) III; axIs1702. axIs1702 [GFP::fbf-2 + unc-119(+)]. Maintain at >22C to maintain transgene expression. Unknown if ed3 is still in background.
JH2883 fbf-1(ok91) II; pgl-1(ct131) him-3(e1147) III; axIs1722. axIs1722 [pie-1p::GFP::H2B::fog-1 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. Unknown if ed3 is still in background.
MG8 csc-1(t1171) dpy-2(e8)/mnC1 [dpy-10(e128) unc-52(e444)] II; him-3(e1147) IV. Heterozygotes are WT and segregate WT, DpyUncs, and Dpys which give only dead eggs. Throws males.
SS2 pgl-1(ct131) him-3(e1147) IV. Temperature sensitive sterility. The sterility has both a maternal and a non-maternal component. Can be maintained indefinitely at low temperatures (16-23 C), with 7-19% sterile offspring. When low-temperature-grown homozygotes are allowed to produce progeny at 25C, the percentage of sterile offspring is 75-85%; at 26C the percentage of sterile offspring is 100%. Throws males.
SS617 pgl-1(ct131) him-3(e1147) unc-24(e138) IV; pgl-3(bn104) dpy-11(e224) V. Throws males. Dpy. Unc. Mostly sterile at all temperatures, but a small fraction are fertile at low temperatures. Maintain at 15C.
SS618 pgl-1(ct131) him-3(e1147) IV; pgl-3(bn104) V. Throws males. Mostly sterile at all temperatures, but a small fraction are fertile at low temperatures. Maintain at 15C.
SS729 pgl-2(bn123) III; pgl-1(ct131) him-3(e1147) IV. Throws males. Maintain at 15C. Sterile at higher temperatures.
TX773 teIs65 II; unc-119(ed3) III; him-3(e1147) IV. teIs65 [pie-1p::GFP::plk-1(PBD) + unc-119(+)] II. Maintain at 25 degrees and by picking the most brightly fluorescing animals to avoid silencing of the transgene. teIs65 contains GFP fused to the PLK-1 protein-binding domain. Derived from injection of pRL1216. Reference: Nishi Y, et al. Development. 2008 Feb;135(4):687-97.
TX796 unc-119(ed3) III; him-3(e1147) IV; teEx321. teEx321[pRL1636 (Pmed-1::GFP::sys-1) pDPmm016]. GFP signal is very weak and can't be see using a dissecting microscope. Very low transmission. Most signal is nuclear and the signal is stronger in the posterior sister, e.g., MSp stronger than MSa, Ep stronger than Ea. Some cortical signal was also detected. Some larvae lack anterior gut or have gaps. Maintain by picking non-Uncs.
TX895 unc-119(ed3) III; him-3(e1147) IV; teIs84 X. teIs84 [end-3p::GFP::H2B + unc-119(+)] X. Him. Superficially wild-type. Integrated E-lineage specific GFP reporter. Reference: Robertson SM, et al. PLoS One. 2014 Sep 2;9(9):e106309.
TX903 teIs90 I; unc-119(ed3) III; him-3(e1147) IV. teIs90 [(pRL1483) pie-1p::GFP::taf-4 + unc119(+)]. Sick at high temperatures. Maintain at 20C, but it will silence after some generations.
TX964 unc-119(ed3) III; him-3(e1147) IV; teIs98. teIs98 [(pRL1450) pie-1p::GFP::sys-1 + unc-119(+)].
VC418 him-3(gk149) IV/nT1 [qIs51] (IV;V). ZK381.1. Homozygous viable deletion balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1 aneuploid progeny, and non-GFP gk149 homozygotes (strongly Him, with small broods and many arrested embryos). nT1[qIs51] homozygotes inviable. Pick GFP WT and check for correct segregation of progeny to maintain. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807