GE2929 |
unc-32(e189) pna-2(t1434)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. |
GE2935 |
let-725(t1440) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. t1440 previously called mel-27. |
GE2940 |
unc-32(e189) pnm-2(t1445)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. |
GE2941 |
unc-32(e189) let-(t1446)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
Heterozygotes are WT and segregate WT, DpySteriles and Uncs which give only dead eggs. This strain was mistakenly called emb-30 in the paper; it is not an emb-30 allele. |
GE2946 |
let-748(t1452) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. |
GE2948 |
unc-32(e189) apo-2(t1454)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. |
GE2952 |
unc-32(e189) pnm-3(t1458)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. |
GE2959 |
unc-32(e189) tbg-1(t1465)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. tbg-1(t1465) previously called sas-3(t1465). |
GE2961 |
unc-32(e189) csg-4(t1467)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. |
GE3023 |
emb-8(t1533) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. |
GE3633 |
unc-32(e189) cyk-4(t1689)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. |
JH2874 |
pgl-1(ct131) him-3(e1147) III; ozIs5. |
ozIs5 [gld-1::GFP + unc-119(+)]. |
JH2877 |
fbf-2(q738) II; pgl-1(ct131) him-3(e1147) III; axIs1722. |
axIs1722 [pie-1p::GFP::H2B::fog-1 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. Unknown if ed3 is still in background. |
JH2883 |
fbf-1(ok91) II; pgl-1(ct131) him-3(e1147) III; axIs1722. |
axIs1722 [pie-1p::GFP::H2B::fog-1 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. Unknown if ed3 is still in background. |
MG8 |
csc-1(t1171) dpy-2(e8)/mnC1 [dpy-10(e128) unc-52(e444)] II; him-3(e1147) IV. |
Heterozygotes are WT and segregate WT, DpyUncs, and Dpys which give only dead eggs. Throws males. |
SS2 |
pgl-1(ct131) him-3(e1147) IV. |
Temperature sensitive sterility. The sterility has both a maternal and a non-maternal component. Can be maintained indefinitely at low temperatures (16-23 C), with 7-19% sterile offspring. When low-temperature-grown homozygotes are allowed to produce progeny at 25C, the percentage of sterile offspring is 75-85%; at 26C the percentage of sterile offspring is 100%. Throws males. |
SS617 |
pgl-1(ct131) him-3(e1147) unc-24(e138) IV; pgl-3(bn104) dpy-11(e224) V. |
Throws males. Dpy. Unc. Mostly sterile at all temperatures, but a small fraction are fertile at low temperatures. Maintain at 15C. |
SS618 |
pgl-1(ct131) him-3(e1147) IV; pgl-3(bn104) V. |
Throws males. Mostly sterile at all temperatures, but a small fraction are fertile at low temperatures. Maintain at 15C. |
SS729 |
pgl-2(bn123) III; pgl-1(ct131) him-3(e1147) IV. |
Throws males. Maintain at 15C. Sterile at higher temperatures. |
TX773 |
teIs65 II; unc-119(ed3) III; him-3(e1147) IV. |
teIs65 [pie-1p::GFP::plk-1(PBD) + unc-119(+)] II. Maintain at 25 degrees and by picking the most brightly fluorescing animals to avoid silencing of the transgene. teIs65 contains GFP fused to the PLK-1 protein-binding domain. Derived from injection of pRL1216. Reference: Nishi Y, et al. Development. 2008 Feb;135(4):687-97. |
TX796 |
unc-119(ed3) III; him-3(e1147) IV; teEx321. |
teEx321[pRL1636 (Pmed-1::GFP::sys-1) pDPmm016]. GFP signal is very weak and can't be see using a dissecting microscope. Very low transmission. Most signal is nuclear and the signal is stronger in the posterior sister, e.g., MSp stronger than MSa, Ep stronger than Ea. Some cortical signal was also detected. Some larvae lack anterior gut or have gaps. Maintain by picking non-Uncs. |
TX895 |
unc-119(ed3) III; him-3(e1147) IV; teIs84 X. |
teIs84 [end-3p::GFP::H2B + unc-119(+)] X. Him. Superficially wild-type. Integrated E-lineage specific GFP reporter. Reference: Robertson SM, et al. PLoS One. 2014 Sep 2;9(9):e106309. |
TX903 |
teIs90 I; unc-119(ed3) III; him-3(e1147) IV. |
teIs90 [(pRL1483) pie-1p::GFP::taf-4 + unc119(+)]. Sick at high temperatures. Maintain at 20C, but it will silence after some generations. |
TX964 |
unc-119(ed3) III; him-3(e1147) IV; teIs98. |
teIs98 [(pRL1450) pie-1p::GFP::sys-1 + unc-119(+)]. |
VC418 |
him-3(gk149) IV/nT1 [qIs51] (IV;V). |
ZK381.1. Homozygous viable deletion balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1 aneuploid progeny, and non-GFP gk149 homozygotes (strongly Him, with small broods and many arrested embryos). nT1[qIs51] homozygotes inviable. Pick GFP WT and check for correct segregation of progeny to maintain. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 |