| GE2685 |
unc-32(e189) csg-8(t1714)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. |
| GE2690 |
let-733(t1683) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. |
| GE2720 |
cta-4(t1562) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. |
| GE2722 |
cyk-1(t1568) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
Heterozygotes are WT and segregate WT, Sterile Dpys and Uncs which give only dead eggs. Throws males. |
| GE2723 |
unc-32(e189) csg-6(t1556)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. |
| GE2727 |
nup-2(t1574) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. |
| GE2730 |
unc-32(e189) lis-1(t1550)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. t1550 pka pnm-1(t1550). |
| GE2929 |
unc-32(e189) pna-2(t1434)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. |
| GE2935 |
let-725(t1440) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. t1440 previously called mel-27. |
| GE2940 |
unc-32(e189) pnm-2(t1445)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. |
| GE2941 |
unc-32(e189) let-(t1446)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
Heterozygotes are WT and segregate WT, DpySteriles and Uncs which give only dead eggs. This strain was mistakenly called emb-30 in the paper; it is not an emb-30 allele. |
| GE2946 |
let-748(t1452) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. |
| GE2948 |
unc-32(e189) apo-2(t1454)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. |
| GE2952 |
unc-32(e189) pnm-3(t1458)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. |
| GE2959 |
unc-32(e189) tbg-1(t1465)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. tbg-1(t1465) previously called sas-3(t1465). |
| GE2961 |
unc-32(e189) csg-4(t1467)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. |
| GE3023 |
emb-8(t1533) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. |
| GE3633 |
unc-32(e189) cyk-4(t1689)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. |
| GE4132 |
unc-32(e189) com-1(t1626)/qC1 [dpy-19(e1259) glp-1(q339)] III. |
Heterozygotes are WT and segregate WT, Dpy Steriles, and Uncs which are Mel with over 99% embryonic lethality. |
| GE68 |
glp-1(e2144) III. |
Sterile at 25C, maintain at 15C. NOTE (11/16/10 - J. Hubbard): This strain is NOT synonymous with glp-1(e2141) as previously reported in Kodoyianni V, Maine EM, Kimble J. (1992) [Molecular basis of loss-of-function mutations in the glp-1 gene of Caenorhabditis elegans. Mol Biol Cell. 3,1199-213. PMID: 1457827]. As reported in WBG article by Dalfó D, Priess J, Schnabel R and Hubbard J. (2010) [glp-1(e2141) sequence correction. The Worm Breeders Gazette. 18-3.], e2144 carries the mutation c2785t in exon 8, leading to the amino acid change L929F, whereas e2141 carries the mutations c2920t and a3610g in exon 8, leading to the amino acid changes R974C and T1204A. |
| GG60 |
glp-1(g60) III. |
Accumulates dead eggs at permissive temperature (15C). Will grow at 20C, but not at 25C. g60 pka emb-33. |
| GS195 |
ncl-1(e1865) unc-36(e251) glp-1(q46) mua-3(ar62) III; qDp3 (III;f). |
Animals with the duplication are WT and segregate WT and larval lethals. Larval muscle dettachment and arrest at about L1 stage in mua-3(ar62) homozygotes. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| GS234 |
mup-4(ar60) ncl-1(e1865) unc-36(e251) glp-1(q46) III; qDp3 (III;f). |
Animals with the duplication are WT and segregate WT and dead eggs. 3-fold muscle dettachment and arrest in 90% of mup-4(ar60) homozygotes; 10% arrest earlier. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| HGA8002 |
glp-1(e2141) III; lynEx1. |
lynEx1 [(pJG01)nhr-80p::nhr-80::GFP + myo-2p::DsRed]. Sterile at 25C. Maintain at 20C or lower. Pick animals with red pharynx to maintain. Lifespan of animals carrying the array is longer than that of glp-1 alone. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599. |
| HGA8004 |
daf-16(mu86) I; glp-1(e2141) III; lynEx1. |
lynEx1 [(pJG01)nhr-80p::nhr-80::GFP + myo-2p::DsRed]. Sterile at 25C. Maintain at 20C or lower. Pick animals with red pharynx to maintain. Lifespan of animals carrying the array is longer than that of daf-16; glp-1 double mutants. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599. |
| HGA8005 |
glp-1(e2141) III; daf-12(rh61rh411) X; lynEx1. |
lynEx1 [(pJG01)nhr-80p::nhr-80::GFP + myo-2p::DsRed]. Sterile at 25C. Maintain at 20C or lower. Pick animals with red pharynx to maintain. Lifespan of animals carrying the array is longer than that of glp-1; daf-12 double mutants. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599. |
| HGA8006 |
glp-1(e2141) III; fat-5 (tm420) V. |
Sterile at 25C. Maintain at 20C or lower. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599. |
| HGA8007 |
glp-1(e2141) III; fat-6 (tm331) IV. |
Sterile at 25C. Maintain at 20C or lower. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599. |
| HGA8008 |
glp-1(e2141) III; fat-7 (wa36) V. |
Sterile at 25C. Maintain at 20C or lower. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599. |
| HGA8009 |
glp-1(e2141) III; fat-5 (tm420) fat-7 (wa36) V. |
Sterile at 25C. Maintain at 20C or lower. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599. |
| HGA8012 |
glp-1(e2141) III; daf-9(rh50) X; lynEx1. |
lynEx1 [nhr-80p::nhr-80::GFP + myo-2p::DsRed]. Sterile at 25C. Maintain at 15-20C. Temperature-sensitve Daf-c. Mig. Pick DsRed+ animals to maintain. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599. |
| HR16 |
mel-23(ct45)/qC1 [dpy-19(e1259) glp-1(q339)] III. |
Heterozygotes are WT and segregate WT and DpySteriles. ct45 homozygotes give only dead eggs at all temperatures. ct45 is ts dominant. Hets give more viable progeny at 15C than 25C. |
| HY463 |
unc-32(e189) pod-1(ye11)/qC1 [dpy-19(e1259) glp-1(q339)] III. |
Heterozygotes are WT and segregate WT, Sterile Dpys and Uncs. Unc worms produce only dead embyros due to strict maternal effect pod-1(ye11) mutation. Embryos from pod-1 homozygous hermaphrodites are osmotically sensitive and exhibit polarity defects. |
| HZ111 |
muIs16 II; sor-1(bp_1)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-5(e1490) V. |
muIs16 [mab-5::GFP]. Larval lethal at L3/L4 stage. Ectopic expression of GFP in the arrested sor-1 mutant homozygotes (L3 stage). NOTE: This allele is published as bp1, but has been curated in WormBase as bp_1 in order to differentiate it from the STS marker bP1. |
| HZ112 |
muIs16 II; sor-1(bp3)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-5(e1490) V. |
muIs16 [mab-5::GFP]. Larval lethal at L3/L4 stage. Ectopic expression of GFP in the arrested sor-1 mutant homozygotes (L3 stage). |
| IP1001 |
nhr-6(lg6001)/qC1 [dpy-19(e1259) glp-1(q339)] III. |
Homozygous nhr-6(lg6001) mutants have defective spermatheca development causing low brood size, abnormally shaped eggs, and occasional Egl. Heterozygotes are WT and segregate WT, semi-sterile WT, and Sterile Dpys. |
| JH2691 |
npp-10(ok467)/qC1 [dpy-19(e1259) glp-1(q339) qIs26] III. |
qIs26 [lag-2::GFP + rol-6(su1006)]. ok467 deletion balanced by glp-1- and dpy-19-marked recombination suppressor. qIs26 was integrated into qC1 and in the process made qC1 homozygous lethal. Heterozygotes are Rollers and GFP+ in the distal tip cell, and segregate WT Rol, lethal qC1 homozygotes, and npp-10 homozygotes (Emb or early Larval lethal). Pick WT GFP+ Rol and check for correct segregation of progeny to maintain. |
| JH2756 |
ect-2(ax751) II; par-3(it71) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; zuIs45 V. |
zuIs45 [nmy-2::NMY-2::GFP + unc-119(+)] V. Temperature-sensitive. Maternal-effect lethal at 25 C. Maintain at 15-20 C. Segregates Unc Mel, non-Unc Mel, non-Unc Ste. Reference: Zonies S, et al. Development. 2010 May;137(10):1669-77. |
| JJ532 |
pie-1(zu154) unc-25(e156)/qC1 [dpy-19(e1259) glp-1(q339)] III. |
Heterozygotes are WT and segregate WT, Unc and DpySteriles. Uncs give only dead eggs. Maintain by picking WT. |
| JK1019 |
dpy-19(e1259) glp-1(q158) III; qDp3 (III;f). |
Animals with the Duplication are WT. Animals which have lost the Duplication are Dpy and Sterile. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| JK1107 |
glp-1(q224) III. |
Temperature sensitive - raise at 15C. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| JK1122 |
dpy-17(e164) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III. |
Heterozygotes are WT and segregate WT, DpyUncs and DpySteriles. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| JK1195 |
lin-12(q269) glp-1(q231)/unc-32(e189) III. |
Heterozygotes are WT and segregate WT, Uncs, and Lags (ts). Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| JK1313 |
lin-12(n941) glp-1(q46)/dpy-19(e1259) unc-69(e587) III. |
Heterozygotes are WT and segregate WT, Dpy(ts) Uncs, and Lag L1 lethals (not ts). Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| JK1438 |
daf-2(m65)/qC1 [dpy-19(e1259) glp-1(q339)] III. |
Heterozygotes are WT and segregate WT, non-conditional dauers, and Sterile Dpys. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| JK1505 |
unc-32(e189) glp-1(e2072) III/eT1 (III;V). |
Heterozygotes are WT and segregate WT, Sterile Unc coilers, Unc-36s (eT1 homozygotes) and dead eggs. Pick WT to maintain. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| JK2533 |
qC1 [dpy-19(e1259) glp-1(q339) qIs26] III/eT1 (III;V). |
qIs26 [lag-2::GFP + rol-6(su1006)]. Throws heterozygous Rollers and Unc eT1 homozygotes. qIs26 was integrated into qC1 and in the process made qC1 homozygous lethal. The distal tip cells are GFP+. It was an integration of qEx233. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| JK289 |
glp-1(e2142) III. |
Temperature sensitive. Maintain at 15C. |
| JK4862 |
glp-1(q46) III/hT2[bli-4(e937) let-?(q782) qIs48] (I;III). |
Homozygous lethal deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP sterile homozygotes. Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Austin J & Kimble J. Cell. 1987 Nov 20;51(4):589-99. |
| JK5008 |
qSi44 II; glp-1(q46) III. |
qSi44 [glp-1::6xMyc6xHis + Cbr-unc-119(+)] II. Homozygous viable. Variable body length. May still carry unc-119(ed3) in the background. Reference: Sorensen EB, et al. A toolkit of tagged glp-1 alleles reveals strong glp-1 expression in the germline, embryo, and spermatheca. microPublication Biology, 2020(06). http://doi.org/10.17912/micropub.biology.000271 |