| AGD1048 |
daf-16(mu86) I; glp-1(e2141) III; uthEx649. |
uthEx649 [rpn-6p::tdTomato + rol-6(su1006)]. Temperature-sensitive. Maintain at 15C; sterile at 25C. Rollers. Pick rollers to maintain array. Reference: Vilchez D, et al. Nature. 2012 Sep 13;489(7415):263-8. |
| AGK650 |
daf-16(mu86) I; zdIs13 IV; armEx257. |
zdIs13 [tph-1p::GFP] IV. armEx257 [dpy-7p::daf-16b::tagRFP + myo-2::GFP]. Pick animals with GFP+ pharynx to maintain. armEx257 rescues the daf-16(mu86) HSN undermigration phenotype. dpy-7p::daf-16b::tagRFP expression is localized to nuclei in hypodermal tissue during the comma, 1.5 and 2-fold stages, becoming cytoplasmic or perinuclear by the 3-fold stage and persisting into adulthood. Reference: Kennedy LM, et al. Cell Rep. 2013 Sep 12;4(5):996-1009. |
| AU147 |
daf-16(mgDf47) I; glp-1(e2141) III. |
Temperature sensitive sterility. Maintain at 15C. |
| AU166 |
daf-16(mgDf47) I; fog-2(q71) V. |
Temperature sensitive. Maintain by mating males & females at 15C. fog-2(q71) is male-female. |
| CF1038 |
daf-16(mu86) I. |
Dauer defective. Short lived. |
| CF1137 |
daf-16(mu86) I; daf-2(e1370) III; muIs61. |
muIs61 [daf-16a::GFP + rol-6(su1006)]. Temperature-sensitive. Maintain at 15 C. Rollers. Reference: Lin K, Hsin H, Libina N, Kenyon C. Nat Genet. 2001 Jun;28(2):139-45. |
| CF1139 |
daf-16(mu86) I; muIs61. |
muIs61 [(pKL78) daf16::GFP + rol-6(su1006)]. muIs61 rescues daf-16(mu86). muIs61 is a gamma-induced insertion of muEx50. muIs61 insertion point has not been mapped. |
| CF1295 |
daf-16(mu86) I; daf-2(e1370) III; muEx108. |
muEx108 [(pKL99-2) daf-16::GFP/daf16bKO + rol-6(su1006)]. Grows okay at 20C. Rollers should form dauers at 25C. Pick Rollers to maintain. |
| CF1308 |
daf-16(mu86) I; muEx116. |
muEx116 [daf-16a::GFP(T54A S240A T242A S314A) + rol-6(su1006)]. Grows at all temperatures. Carries extrachromosomal array. Pick Rollers to maintain. |
| CF1330 |
daf-16(mu86) I; muEx128. |
muEx128 [(pKL79) daf-16a::GFP) + rol-6(su1006)]. Grows okay at 20C. Pick Rollers to maintain. |
| CF1371 |
daf-16(mu86) I; muEx151. |
muEx151 [(pKL106-8) daf-16aAM::GFP/bKO + rol-6(su1006)]. Grows okay at 20C. Pick Rollers to maintain. |
| CF1380 |
daf-16(mu86) I; daf-2(e1370) III; muEx158. |
muEx158 [daf-16cAM::GFP + sur-5p::GFP] (AM = AKT-site mutant). Pick GFP+ worms to maintain. Sterile at 25C; grow at 20C or less. muEx158 contains GFP-tagged daf-16 c isoform (described as a1 isoform in Lin, et al. Nat Genet. 2001) with 4 Ser/Thr residues mutated to Ala, which completely rescues dauer formation and partially restores longevity of daf-16; daf-2 double mutants. Reference: Lin K, et al. Nat Genet. 2001 Jun;28(2):139-45. |
| CF1407 |
daf-16(mu86) I; muIs71 X. |
muIs71 [(pKL99) daf-16ap::GFP::daf-16a(bKO)) + rol-6(su1006)]. Grows okay at 20C. Spontaneous integrant. Rollers. |
| CF1442 |
daf-16(mu86) I; daf-2(e1370) III; muEx169. |
muEx169 [unc-119p::GFP::daf-16 + rol-6(su1006)]. Pick Rollers to maintain. May grow better at 15C. |
| CF1449 |
daf-16(mu86) I; daf-2(e1370) III; muEx176. |
muEx176 [daf-16p::GFP::daf-16 + rol-6(su1006)]. Pick rollers to maintain -- Low transmission rate! Maintain at 15C. Forms dauers at 25C. Reference: Lin K, et al. Nat Genet. 2001 Jun;28(2):139-45. |
| CF1514 |
daf-16(mu86) I; daf-2(e1370) III; muEx211. |
muEx211[pNL213(ges-1p::GFP::daf-16) + rol-6(su1006)]. Grows at 15C (probably also at 20C). Pick Rollers to maintain. |
| CF1515 |
daf-16(mu86) I; daf-2(e1370) III; muEx212. |
muEx212[pNL212(myo-3p::GFP::daf-16) + rol-6(su1006)]. Grows at 15C (probably also at 20C). Pick Rollers to maintain. |
| CF1588 |
daf-16(mu86) I; daf-2(e1370) III; muIs84. |
muIs84 [(pAD76) sod-3p::GFP + rol-6(su1006)]. Dim green expression in head, tail and around vulva. Daf-d. Can grow at 20C. |
| CF1595 |
daf-16(mu86) I; daf-2(e1370) III; muEx227. |
muEx227 [(pNL213) ges-1p::GFP::daf-16) + rol-6(su1006)]. Pick Rollers to maintain. |
| CF1660 |
daf-16(mu86) I; daf-2(e1370) III; muIs84; muEx211. |
muIs84 [(pAD76) sod-3p::GFP + rol-6(su1006)]. muEx211 [ges-1p::daf-16::GFP + rol-6(su1006)]. Pick Rollers to maintain. Partial rescue of lifespan phenotype. Some animals show variable daf-16 expression in the intestine. Grows okay at 15C. [NOTE: muEx211 is quite unstable. Be sure to pick Rollers to avoid losing the array.] |
| CF1700 |
daf-16(mu86) I; mes-1(bn7) X; muEx248. |
muEx248 [(pNL209) daf-16::GFP::daf-16(cDNA) + podr-1::RFP]. Pick green (body) / red (head neurons) animals. Transmission efficiency ~50%. Can be grown at 20C with some sterility (30-50%). The higher the temperture, the greater the sterility. |
| CF1724 |
daf-16(mu86) I; daf-2(e1370) III; muIs105. |
muIs105 [daf-16p::GFP::daf-16 + rol-6(su1006)]. Rollers; Rol phenotype is not always evident). Integrated line derived from CF1449. Maintain at 15C. Forms dauers at 25C. Reference: Lin K, et al. Nat Genet. 2001 Jun;28(2):139-45. |
| CF1827 |
daf-16(mu86) I; daf-2(e1370) III; muEx268. |
muEx268 [ges-1p::GFP::daf-16(cDNA) + odr-1::RFP]. daf-16 GFP expressed in intestine. Partial rescue of lifespan phenotype. Grows okay at 15C. Pick RFP to maintain. |
| CF1874 |
daf-16(mu86) I; muIs84. |
muIs84 [(pAD76) sod-3p::GFP + rol-6(su1006)]. Green expression in head, tail and around vulva. |
| CF1880 |
daf-16(mu86) I; glp-1(e2141) III. |
Sterile at 25C; grow at 20C or less. glp-1(e2141) longevity suppressed by daf-16(mu86). |
| CF1935 |
daf-16(mu86) I; glp-1(e2141) III; muIs109. |
muIs109 [daf-16p::GFP::DAF-16 cDNA + odr-1p::RFP]. Sterile at 25C; grow at 20C or less. |
| CF2005 |
daf-16(mu86) I; daf-2(e1370) III; muIs120. |
muIs120 [ges-1p::GFP::daf-16 + rol-6(su1006)]. Maintain at 15-20C. Rollers. Long-lived. Gamma irradiation-induced integration of muEx211. Rescues daf-16a1/c in the intestine (described in Libina et al, 2003). Reference: Zhang P, et al. Cell Metab, 2013. 17(1): p. 85-100. |
| CF2060 |
daf-16(mu86) I; muEx158. |
muEx158 [daf-16cAM::GFP + sur-5p::GFP] (AM = AKT-site mutant). Pick GFP+ worms to maintain. Sterile at 25C; grow at 20C or less. muEx158 contains GFP-tagged daf-16 c isoform (described as a1 isoform in Lin, et al. Nat Genet. 2001) with 4 Ser/Thr residues mutated to Ala, which rescues daf-16 mutants from defective dauer formation and also partially rescues longevity defect of daf-16; glp-1 double mutants. Reference: Berman JR, & Kenyon C. Cell. 2006 Mar 10;124(5):1055-68. |
| CF2061 |
daf-16(mu86) I; glp-1(e2141) III; muEx158. |
muEx158 [daf-16AM::GFP + sur-5p::GFP]. Pick GFP+ worms to maintain. Sterile at 25C; grow at 20C or less. |
| CF2093 |
daf-16(mu86) I; daf-2(e1370) III; muIs131. |
muIs131 [unc-119p::GFP::daf-16 + rol-6(su1006)]. Maintain at 15-20C. Rollers. Modestly long-lived. Gamma irradiation-induced integration of muEx184. Rescues daf-16a1/c in neurons (described in Libina et al, 2003). Reference: Zhang P, et al. Cell Metab, 2013. 17(1): p. 85-100. |
| CF2102 |
daf-16(mu86) I; daf-2(e1370) III; muIs126. |
muIs126 [myo-3p::GFP::daf-16 + rol-6(su1006)]. Maintain at 15-20C. Rollers. Gamma irradiation-induced integration of muEx215. Rescues daf-16a1/c in muscles (described in Libina et al, 2003). Reference: Zhang P, et al. Cell Metab, 2013. 17(1): p. 85-100. |
| CF2135 |
daf-16(mu86) kri-1(ok1251) I; glp-1(e2141) III; muIs109. |
muIs109 [daf-16p::GFP::DAF-16 cDNA + odr-1p::RFP]. Sterile at 25C; grow at 20C or less. |
| CF2247 |
daf-16(mu86) I; glp-1(e2141) III; muEx248. |
muEx248 [daf-16::GFP::DAF-16 cDNA + odr-1p::RFP]. Sterile at 25C; grow at 20C or less. Pick RFP+/GFP+ animals to maintain. |
| CF2248 |
daf-16(mu86) I; glp-1(e2141) III; daf-12(rh61rh411) X; muEx158. |
muEx158 [daf-16AM::GFP + sur-5p::GFP]. Pick GFP+ worms to maintain. Sterile at 25C; grow at 20C or less. |
| CF2263 |
daf-16(mu86) I; glp-1(e2141) III; daf-9(rh50) X; muEx158. |
muEx158 [daf-16AM::GFP + sur-5p::GFP]. Pick GFP+ worms to maintain. Sterile at 25C; grow at 20C or less. |
| CF2265 |
daf-16(mu86) kri-1(ok1251) I; glp-1(e2141) III; muEx158. |
muEx158 [daf-16AM::GFP + sur-5p::GFP]. Pick GFP+ worms to maintain. Sterile at 25C; grow at 20C or less. |
| CF2278 |
daf-16(mu86) I; glp-1(e2141) III; daf-12(rh61rh411) X; muEx248. |
muEx248 [daf-16p::GFP::DAF-16 cDNA + odr-1p::RFP]. Sterile at 25C; grow at 20C or less. Pick RFP+/GFP+ animals to maintain. |
| CF2288 |
daf-16(mu86) I; glp-1(e2141) III; daf-9(rh50) X; muEx248. |
muEx248 [daf-16p::GFP::DAF-16 cDNA + odr-1p::RFP]. Sterile at 25C; grow at 20C or less. Pick RFP+/GFP+ animals to maintain. |
| CF2570 |
daf-16(mu86) I; daf-2(e1370) III; muIs142. |
muIs142 [ges-1p::GFP::daf-16(cDNA) + odr-1p::RFP]. Maintain at 15-20C. Gamma irradiation-induced integration of muEx268. Rescues daf-16a1/c in the intestine (described in Libina et al, 2003). Reference: Zhang P, et al. Cell Metab, 2013. 17(1): p. 85-100. |
| CY397 |
daf-16(mg242) I; sqt-1(sc13) age-1(mg109) II. |
Sqt phenotype. The daf-16(mg242) allele is a dominant suppressor of age-1(mg109) daf-c phenotype. mg242 is a nonsense mutation Trp220Amb. |
| CY398 |
daf-16(mg255) I; sqt-1(sc13) age-1(mg109) II. |
Sqt phenotype. The daf-16(mg255) allele is a dominant suppressor of age-1(mg109) daf-c phenotype. mg255 is a nonsense mutation Try144Amb. |
| DR1309 |
daf-16(m26) I; daf-2(e1370) III. |
|
| DR1408 |
daf-16(m26) I; age-1(m333) II. |
Egl phenotype, viable. Lethality and Daf-c phenotype of m333 suppressed by daf-16 (m333 alone is a maternal-effect non-conditional dauer constitutive). age-1(m333) pka daf-23(m333). |
| DR195 |
dpy-5(e61) daf-16(m26) I. |
Dauer defective. Leaky. Dpy. |
| DR210 |
dpy-5(e61) daf-16(m26) unc-75(e950) I. |
Dauer defective. Dpy. Unc. Leaky. |
| DR211 |
daf-16(m26) unc-75(e950) I. |
Dauer defective. Unc. |
| DR2203 |
daf-16(m26) I; rrf-3(b26) liv-2(m882) II. |
daf-d. Maintain at 15C. |
| DR2208 |
daf-16(m26) I; rrf-3(b26) II; liv-4(m872) V. |
daf-d on starvation. Sterile at 25C. Maintain at 15C. Mild Unc. |
| DR234 |
dpy-5(e61) daf-16(m27) I. |
Dauer defective. Dpy. |
| DR26 |
daf-16(m26) I. |
Dauer defective-leaky. Somewhat small. Suppresses daf-2. |