||unc-85(e1414) bli-2(e768) dpy-10(e128) II.
||Unc and Dpy. Not Blistered: dpy-10 suppresses the appearance of the blisters.
||Bursae abnormal. Lineage abnormal. Unc-kinky backing. Unc-lurches forward. Egl. M-MATING-NO SUCCESS. Rec'd new stock from J. Hodgkin 12/4/00.
||mup-1(e2430) lin-31(n301) unc-85(e1414) II.
||mup-1(e2430) is a temperature sensitive two-fold arrest lethal. Maintain at 15C. Easily scored, at restrictive temperature they look like tiny little croissants. Muv and Unc.
||unc-85(e1414) II; him-8(e1489) IV.
||Unc hermaphrodites and males. Mate have variably abnormal tail anatomy. ME 0.
||evl-2(ar119)/unc-85(e1414) dpy-10(e128) II.
||Heterozygotes are WT and segregate WT, DpyUncs and Steriles which have an everted Vulva. CGC rec'd new stock from Iva Greenwald 5/2000. The unc-85 dpy-10 chromosome has been lost from this stock. For some reason it was difficult to maintain as a heterozygote. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
||lin-31(n301) unc-85(e1414) II.
||F10G7.3. External left primer: TTCGAAATCGATTGAAACCC. External right primer: GCTCGAGAGGCTGCTTTAGA. Internal left primer: TCCGTTCGAAATTTCCTGTT. Internal right primer: CCCCGTGTCTTTCATTGATT. Internal WT amplicon: 2106 bp. Deletion size: 1708 bp. Deletion left flank: TGGAAAAATAAATAAATAAATACGAAGAAG. Deletion right flank: AGTAAAGAAATTGATTTAAAAAGAAAATAA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807