Gene Information: unc-4

Nameunc-4 View on WormBase
Species C. elegans
SequenceF26C11.2
Genetic positionII:1.77 +/- 0.006 cM
Genomic positionII: 9897751..9900376

Strains carrying this gene

Strain Genotype Description
AG150 apc-1(ar104)/unc-4(e120) bli-1(e769) II. Heterozygotes are WT and segregate WT, UncBli, and Steriles which have an everted vulva. ar104 previously called evl-22 and mat-2.
AG168 fzy-1(av15) unc-4(e120) II. Unc. Gain-of-function allele of fzy-1.
AG226 rol-6(e187) unc-4 (e120)/mnC1 [dpy-10(e128) unc-52(e444) nIs190 let-?] II; him-8(e1489) IV. nIs190 [myo-2::GFP]. Him. Heterozygotes are wild-type GFP+ and segregate WT GFP+ heterozygotes, Rol Uncs, dead embryos, and males. nIs190 [myo-2::GFP] integrated in or near mnC1. Approx 0.5% recombination seen between nIs190 and mnC1. Fails to complemement all markers on mnC1.
AH286 unc-4(e120) ect-2(zh8) II; gap-1(ga133) X. Muv and Unc. Semi-dominant mutation in ect-2 (previously called let-21).
AH346 dep-1(zh34) unc-4(e120) II; lip-1(zh15) IV. Pvl and weak Muv. Transformation of secondary to primary vulval cell fates.
AN170 aff-1(ty4) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. Heterozygotes are WT and segregate WT (heterozygotes), Unc-4 worms which are strong Egl (ty4 homozygotes), and paralyzed Dpy Uncs (mnC1 homozygotes).
AT28 kyIs140 I; srf-6(yj13) unc-4(e120) II. kyIs140 [str-2::GFP + lin-15(+)] I. Kinker; can't back up. srf-6 mutants express str-2::GFP in both AWC neurons (2AWC ON phenotype; wild-type phenotype is 1AWC ON): check for this phenotype to avoid reversion of srf-6(yj13). srf-6 mutants were originally identified by binding of an L1-specific antibody in later larval stages (L1-L4).
AT30 kyIs140 I; nsy-1(ok593) unc-4(e120) II. kyIs140 [str-2::GFP + lin-15(+)] I. nsy-1(ky593) has no visible phenotype, but can be tracked by linked Unc-4 phenotype (Kinker, can't back up). str-2::GFP is expressed in both AWC neurons.
CB120 unc-4(e120) II. Uncoordinated. May contain a weak daf-2 mutation (sa875). See Ailion and Thomas in Genetics 165: 127-144 2003.
CB2597 tra-2(e1098)/dpy-10(e128) unc-4(e120) II. Heterozygotes are WT and segregate WT, DpyUnc and males. Maintain by picking WT hermaphrodites.
CB2754 tra-2(e1095)/dpy-10(e128) unc-4(e120) II. Heterozygotes are WT and segregate WT, DpyUnc and males. Maintain by picking WT.
CB2830 tra-2(e1209)/dpy-10(e128) unc-4(e120) II. Balances well. Transformer weak. Heterozygotes are WT and segregate WT, DpyUnc and males. Maintain by picking WT.
CB3970 unc-4(e120) bli-1(e769) II. Uncoordinated and Blistered.
CB4050 tra-2(e2046e2115) unc-4(e120) II; sup-7(st5) X. Phenotypically wildtype XX hermaphrodite at 20C; poor viability and fertility at 25C. Rare XO animals are hermaphrodite due to suppression of amber mutation e2115 and consequent transformation of XO due to tra-2(e2046) gain-of-function. Reference: Hodgkin & Albertson (1995) PMID: 8647390.
CB4330 unc-4(e2309) II. Reference:
CB5023 tra-2(e2046e253) unc-4(e120) / + + II; dpy-26(n199) IV. [XO females, dpy-26 XO males] Obligate XO male/female strain, propagate by crossing. Low fertility. Fertile XO females and XO males; inviable XX embryos and larvae. Reference: Strain 7 Hodgkin (2002) PMID: 12399387.
CB5310 unc-4(e120) II; lon-2(e678)^lon-2(e678) X. Attached X strain with autosomal unc-4 marker, which facilitates test-crosses. Uncoordinated Long hermaphrodites, reduced fertility with many X^X X^X unhatched eggs. Reference: Hodgkin & Albertson (1995) PMID: 8647390.
CE1857 ect-2(e1778)/unc-4(e120) sqt-1(sc13) II. Heterozygotes are WT and segregate WT, Roller Uncs, and ect-2 homozygotes (sterile Uncs which reach adulthood, sometimes giving polynucleate oocytes). ect-2 pka let-21. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
CGC17 unc-4(e120)/mT1 [umnIs6] II; dpy-17(e164)/mT1 [dpy-10(e128)] III. umnIs6 [eft-3p::NLS::tdTomato + HygroR, III:~5753000 (intergenic)] II. Heterozygotes are WT with dim red fluorescence, and segregate WT with dim red fluorescence, arrested mT1 aneuploids, sterile Dpys (mT1 homozygotes with more intense red fluorescence), and DpyUnc with no red fluorescence. Pick WT with dim red fluorescence and check for correct segregation of progeny to maintain.
CGC43 unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444) umnIs32] II. umnIs32 [myo-2p::GFP + NeoR, II: 11755713 (intergenic)] II. Hets are WT GFP+ and segregate WT GFP+, Unc-4 (GFP-) and paralysed DpyUnc GFP+ (mnC1 homozygotes). Maintain by picking WT GFP+. Derived by insertion of myo-2p::GFP transgene into mnC1 balancer in parental strain SP127 using CRISPR/Cas9.
CGC44 mIn1 [dpy-10(e128) umnIs33]/unc-4(e120) II. umnIs33 [myo-2p::GFP + NeoR, II: 11755713 (intergenic)] II. Heterozygotes are wild-type GFP+, and segregate wild-type GFP+, Unc-4 non-GFP, and Dpy GFP+ mIn1 homozygotes. Maintain by picking wild-type GFP+ and check for correct segregation of progeny to maintain. Derived by insertion of myo-2p::GFP transgene into mIn1 balancer in parental strain DR1785 using CRISPR/Cas9.
CGC45 unc-4(e120)/mT1 [umnIs34] II; mT1 [dpy-10(e128)]/dpy-17(e164) III. umnIs34 [myo-2p::GFP + NeoR, III: 8856215 (intergenic)] II. Heterozygotes are wild-type GFP+, and segregate wild-type GFP+, DpyUnc non-GFP, sterile Dpy GFP+ mT1 homozygotes, and large numbers of arrested aneuploid embryos. Maintain by picking wild-type GFP+ and check for correct segregation of progeny to maintain. Derived by insertion of myo-2p::GFP transgene into mT1 balancer in parental strain DR1832 using CRISPR/Cas9.
CGC48 unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444) umnIs37] II. umnIs37 [myo-2p::mKate2 + NeoR, II: 11755713 (intergenic)] II. Hets are WT mKate2+ and segregate WT mKate2+, Unc-4 (no red fluorescence) and paralysed DpyUnc mKate2+ (mnC1). Maintain by picking WT mKate2+. Derived by insertion of myo-2p::mKate2 transgene into parental strain SP127 using CRISPR/Cas9.
CGC53 mIn1 [dpy-10(e128) umnIs43]/unc-4(e120) II. umnIs43 [myo-2p::mKate2 + NeoR, II: 11755713 (intergenic)] II. Heterozygotes are wild-type mKate2+, and segregate wild-type mKate2, Unc-4 non-mKate2, and Dpy mKate2+ mIn1 homozygotes. Maintain by picking wild-type mKate2+ and check for correct segregation of progeny to maintain. Derived by insertion of myo-2p::mKate2 transgene into mIn1 balancer in parental strain DR1785 using CRISPR/Cas9.
CGC65 mT1/unc-4(e120) II; mT1 [dpy-10(e128) umnIs51]/dpy-17(e164) III. umnIs51 [myo-2p::mKate2 + NeoR, III: 11755713 (intergenic)] II. Heterozygotes are wild-type mKate2+, and segregate wild-type mKate2+, DpyUnc non-mKate2, sterile Dpy mKate2+ mT1 homozygotes, and large numbers of arrested aneuploid embryos. Maintain by picking wild-type mKate2+ and check for correct segregation of progeny to maintain. Derived by insertion of myo-2p::mKate2 transgene into mT1 balancer in parental strain DR1832 using CRISPR/Cas9.
CGC66 unc-4(e120)/mT1 [umnIs52] II; mT1 [dpy-10(e128)]/dpy-17(e164) III. umnIs52 [myo-2p::mKate2 + NeoR, III: 8856215 (intergenic)] II. Heterozygotes are wild-type mKate2+, and segregate wild-type mKate2+, DpyUnc non-mKate2, sterile Dpy mKate2+ mT1 homozygotes, and large numbers of arrested aneuploid embryos. Maintain by picking wild-type mKate2+ and check for correct segregation of progeny to maintain. Derived by insertion of myo-2p::mKate2 transgene into mT1 balancer in parental strain DR1832 using CRISPR/Cas9.
CGC68 mT1/unc-4(e120) II; mT1 [dpy-10(e128) umnIs54]/dpy-17(e164) III. umnIs54 [myo-2p::GFP + NeoR, III: 11755713 (intergenic)] II. Heterozygotes are wild-type GFP+, and segregate wild-type GFP+, DpyUnc non-mGFP, sterile Dpy GFP+ mT1 homozygotes, and large numbers of arrested aneuploid embryos. Maintain by picking wild-type GFP+ and check for correct segregation of progeny to maintain. Derived by insertion of myo-2p::GFP transgene into mT1 balancer in parental strain DR1832 using CRISPR/Cas9.
DM3414 unc-4(e120) let-268(ra414)/mnC1 [dpy-10(e128) unc-52(e444)] II. Heterozygotes are WT and segregate WT, paralyzed Dpys and lethals.
DR103 dpy-10(e128) unc-4(e120) II. DpyUnc. [NOTE: Likely contains background mrt mutation (re47) (D. Reiner). Segregates males, sickly animals, and dark steriles growing progessively worse in subsequent generations. (D. Reiner & S. Ahmed, 2011)]
DR1218 mnC1 [dpy-10(e128) unc-52(e444)]/unc-4(e120) let-265(mn188) II. Heterozygotes are WT and segregate WT, DpyUnc, and Lethal Unc-4 (lethal mid-larval). Maintain by picking WT.
DR1720 unc-4(e120) daf-19(m86) II. Unc. Dauer constitutive.
DR1785 mIn1 [dpy-10(e128)]/unc-4(e120) II. WT phenotype. Segregates WT, homozygous Dpy-10 mIn1 and homozygous Unc-4 hermaphrodites. Recombination in this interval is suppressed, and recombinant animals have not been detected in this stock. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to Don Riddle. mIn1 pka mC6.
DR1809 mIn1 [dpy-10(e128) let-?(m727)]/unc-4(e120) II. Heterozygotes are WT and segregate WT, unc-4, and lethal mIn1 homozygotes. Pick WT and check for correct segregation of progeny.
DR1832 mT1/unc-4(e120) II; mT1 [dpy-10(e128)]/dpy-17(e164) III. WT phenotype. Segregates WT, sterile Dpy mT1 homozygotes, Unc-4;Dpy-17 and large numbers of arrested aneuploid embryos. Pick WT and check for correct segregation of progeny to maintain. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to Don Riddle.
DR2054 mIn1 [unc-4(e120) dpy-10(e128)]/let-552(e2542) rol-1(e91) II. Heterozygotes are WT and segregate WT, DpyUnc [mIn1(mc6) homozygotes], and two-fold arrest Let Rol progeny. Well balanced. Male stock. Cross WT males to WT hermaphrodites and check for correct segregation of progeny.
DR2075 mIn1 [unc-4(e120)]/let-552(e2542) rol-1(e91) II. Heterozygotes are WT and segregate WT, Lethal Rollers (arrested 2-fold hatchlings), and Unc-4 mIn1(mC6) homozygotes. Pick WT and check for correct segregation of progeny.
DR2078 mIn1 [dpy-10(e128) mIs14]/bli-2(e768) unc-4(e120) II. WT gross phenotype, with GFP semi-dominantly expressed in 4-60 cell embyros, pharyngeal muscle and gut. Segregates WT, brighter Dpy GFP mIn1 homozygotes and non-GFP bli-2 unc-4 homozygotes. Pharyngeal and gut GFP is easily seen in a UV dissecting microscope; early embryonic signal requires higher magnification. mIs14 occasionally crosses off mIn1[dpy-10], apparently by double recombination. Pick WT, check for GFP and check for correct segregation of progeny to maintain. mIs14 is ccEx9747 integrated into mIn1[dpy-10]. This is a three-construct element containing myo-2 and pes-10 promoters and a gut enhancer fused individually to GFP coding sequence.
DR2102 mIn1 [rol-1(e91)]/let-552(e2542) unc-4(e120) II. Heterozygotes are WT and segregate WT, Lethal Uncs and Roller mIn1 homozygotes. let-552 unc-4 chromosome is well balanced. Rol does not express until late L4/early adult. Pick WT and check for correct segregation of progeny.
DR2150 mIn1 [rol-1(e91) dpy-10(e128)]/let-552(e2542) unc-4(e120) II. Heterozygotes are WT and segregate WT, DpyRol mIn1 homozygotes and 2-fold arrest Unc larvae. Male stock; mate WT males and WT hermaphrodites to maintain.
DR518 rol-6(su1006) unc-4(e120) II. RollerUnc.
DZ393 mab-3(e1240) unc-4(e120) ref-1(ez11) II; him-8(e1489) IV. Unc (cannot back). WT male tail.
EK224 cmIs6 I; unc-4(e120) II. cmIs6 [(pBR104) mbk-1::GFP + pNC4.21].
EM172 unc-4(e120) II; him-5(e1490) V; lin-32(bx46) X. Missing Rays. Unc.
EW33 unc-4(e120) pvl-4(ga96) II. Egl. Pvl. Misshapen larvae (some lethal). Unc.
GD211 rol-6(e187) hlh-6(tm299) unc-4(e120) II.
GE1708 dpy-2(e8) unc-4(e120) II. DpyUnc.
GE1709 vab-9(e1744) unc-4(e120) II; him-5(e1490) V. Unc. Slightly Dpy. Tail whip knobbed at all stages except adult male (adult male tail tip slightly swollen). Variably Egl.
GE1710 rol-6(e187) unc-4(e120) II. Roller Unc.
GE1711 dpy-2(e8) vab-9(e1744) unc-4(e120) II. Dpy. Unc. Tail whip knobbed at all stages except adult male (adult male tail tip slightly swollen).
GE1712 vab-9(e1744) rol-6(e187) unc-4(e120) II. Unc. Roller. Tail whip knobbed at all stages except adult male (adult male tail tip slightly swollen).