| AG50 |
daf-7(e1372) dpy-1(e1) III. |
Maintain at 15C. Temperature-sensitive dauer constitutive: 100% dauers at 25C, leaky at 20C. Dpy. Crowds. |
| CB1372 |
daf-7(e1372) III. |
Temperature sensitive dauer constitutive. Crowds. 100% dauers at 25C. Leaky at 20C. Growth slow. M-MATING+LOW TEMPERATURE ONLY. |
| DA2202 |
daf-7(e1372) III; adEx2202. |
adEx2202 [gpa-4p::daf-7 + rol-6p::GFP]. Rescues Daf-c. Maintain by picking GFP+. Reference: You et al (2008) Cell Metab 7(3):249-57. |
| DR1228 |
unc-45(e286) daf-7(e1372) dpy-1(e1) III. |
Dpy. Temperature sensitive dauer constitutive (leaky). Temperature sensitive Unc. At 15C, Dpy adults and some dauers. At 25C, DpyUnc dauers and some DpyUnc adults (leakiness varies: 60-95% dauers). |
| DR133 |
daf-7(e1372) dpy-1(e1) unc-32(e189) III. |
Temperature sensitive dauer constitutive. 100% dauers at 25C. Dpy. Unc-coiler. Reference strain for daf-7 complementation. |
| DR1799 |
daf-7(n696)/unc-45(st604) III. |
WT phenotype. Segregates WT, constitutive dauers and ts mild Uncs. Uncs are maternal-effect lethal: F1 Uncs from a heterozygote produce only arrested 2-fold stage progeny that hatch. Grow at 22.5C (n696 produces nearly 100% dauers at this temperature). |
| DR497 |
unc-13(e450) I; daf-7(m62) III; sup-7(st5) X. |
Both unc-13 and daf-7 are suppressed by sup-7. sup-7 should be grown between 22.5C and 24C. |
| DR62 |
daf-7(m62) III. |
Temperature sensitive dauer constitutive. 100% dauers at 25C. Some dauers (3.5%) at 15C. |
| FK181 |
ksIs2. |
ksIs2 [daf-7p::GFP + rol-6(su1006)]. Rollers. |
| JR1763 |
wcDf1 dpy-1(e1)/daf-7(e1372) par-2(it46) III. |
At 25C, heterozygotes are WT and segregate WT, dead eggs and Dauers (which will give only dead eggs if they exit dauer). e1372 and it46 are both temperature sensitive. |
| KK114 |
daf-7(e1372) par-2(it5) III. |
Temperature sensitive-grow at 15C. Par at 25C. Strict maternal effect lethal. |
| LV15 |
unc-45(st601)/daf-7(e1372) par-2(it46) III. |
st601 is a non-conditional two-fold arrest lethal. Maintain at 25C to see daf-7 par-2 segregants (Daf-7(+) escapers will be Par). WT heterozygotes segregate WT, DafPar, 1/4 lethal eggs and two-fold arrest hatchlings. [3/97: dauers are not giving dead eggs-they are giving more dauers. The par-2 mutation may be lost.] |
| LV18 |
unc-45(wc1) dpy-1(e1)/daf-7(e1372) par-2(it46) III. |
Maintain at 25C. At 25C, heterozygotes are WT and segregate WT, Dauers (dauer escapers will be Par and give only dead eggs), and DpyUcs which arrest as dead eggs (range from twitching multicellulars to 3-folds that hatch). [There is a greater percentage of hatchlings when the mother is heterozygous (wc1 dpy-1/+). There may also be the possibility of near complete maternal rescue (near full-sized, sterile Dpys), but this has not been routinely observed in the balanced strain (as opposed to wc1 dpy-1/+).] Maintain by picking WT at 25C and scoring for correct segregation of progeny. [3/97: The dauers are not giving dead eggs-they are giving other dauers. Appears that the Par mutation is no longer present.] |
| LV19 |
unc-45(wc2) dpy-1(e1)/daf-7(e1372) par-2(it46) III. |
Maintain at 25C. At 25C, heterozygotes are WT and segregate WT, Dauers (escapers will be Par and give only dead eggs) and DpyUncs which are dead eggs (a range of embryonic lethality from multicellular twitchers to 3-folds that do not hatch). Maintain by picking WT at 25C and scoring for correct segregation of progeny. [3/97: the dauers are giving dead eggs.] |
| OH14946 |
ieSi57 II; daf-7(e1372) III; daf-3(ot877[daf-3::TagRFP-T::3xFlag::AID]) X. |
ieSi57 [eft-3p::TIR1::mRuby + unc-119(+)] II. Maintain at 15C. Temperature-sensitive dauer constitutive. CRISPR/Cas9-engineered AID conditional daf-3 allele in daf-7(e1372) background with ubiquitous TIR1 expression. Reference: Aghayeva et al., submitted |
| OH14984 |
daf-7(e1372) III; daf-12(ot874[daf-12::TagRFP::AID]) X. |
Temperature sensitive dauer constitutive. Maintain at 15C. CRISPR/Cas9-engineered AID conditional daf-12 allele in daf-7(e1372) background (TIR1-less control). Reference: Aghayeva et al., submitted |
| OH14986 |
ieSi57 II; daf-7(e1372) III; daf-12(ot874[daf-12::TagRFP-T::AID]) X. |
ieSi57 [eft-3p::TIR1::mRuby + unc-119(+)] II. Maintain at 15C. Temperature-sensitive dauer constitutive. CRISPR/Cas9-engineered AID conditional daf-12 allele in daf-7(e1372) background with ubiquitous TIR1 expression. Reference: Aghayeva et al., submitted |
| OH14989 |
ieSi60 II; daf-7(e1372) III; daf-12(ot874[daf-12::TagRFP-T::AID]) X. |
ieSi60 [myo-2p::TIR1::mRuby + unc-119(+)] II. Maintain at 15C. Temperature-sensitive dauer constitutive. Pharyngeal muscle-specific depletion of DAF-12 in the presence of auxin. Reference: Aghayeva et al., submitted |
| OH15913 |
daf-7(e1372) III; daf-12(ot874[daf-12::TagRFP::AID]) X; otIs730. |
otIs730 [UPN::TIR1::mTur2 + inx-6(prom18)::TagRFP]. Temperature sensitive dauer constitutive. Maintain at 15C. UPN (Ultra Pan-Neuronal) promoter contains four short pan-neuronal promoters fused together (unc-11::rgef-1::ehs-1::ric-19). Panneuronal depletion of DAF-12 in the presence of auxin. Reference: Aghayeva et al., submitted |
| PJ1196 |
daf-7(m62) III; ccIs55 V. |
ccIs55 [unc-54::lacZ + sup-7(st5)] V. Maintain at 15C. Temperature-sensitive; constitutive dauer formation at 25C. NOTE: m62 is an amber allele of daf-7, which is well suppressed by sup-7 in ccIs55 at 16C. |
| PJ1199 |
daf-7(m62) III; ccIs55 V. |
ccIs55 [unc-54::lacZ + sup-7(st5)] V. Maintain at 15C. Constitutive dauer formation at 25C. |
| RB2302 |
daf-7(ok3125) III. |
B0412.2 Homozygous. Maintain at 15C. Outer Left Sequence: cttccttctttccctcccac. Outer Right Sequence: ttgtgacaatcggaagtgga. Inner Left Sequence: gcttatccggatttgacgaa. Inner Right Sequence: catttcttggcgatcattcc. Inner Primer PCR Length: 1162. Deletion size: about 600 bp. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 |
| TY3753 |
daf-7(e1372) III; cuIs2 IV. |
cuIs2 [myo-2c:: GFP + rol-6(su1006)]. Weak Rollers. Dauer constitutive at higher temperatures. Maintain at 15C. |
| TY3862 |
daf-7(e1372) III; cuIs5. |
cuIs5 [myo-2c:: GFP + rol-6(su1006)]. Daf-c. Maintain at 15C. Rollers. GFP+ in pharynx. Reference: Reiner DJ, et al. (2008) Current Biology 18(15):1101-9. |