| AF1 |
+/szT1 [lon-2(e678)] I; dpy-8(e1321) unc-3(e151)/szT1 X. |
Heterozygotes are WT and segregate WT, DpyUnc, dead eggs and Lon males. Maintain by picking WT. |
| AV311 |
dpy-18(e364) unc-3(e151) meT7 (III;X;IV). |
Dpy. Unc. meT7 is an end-to-end-to-end fusion of chromosomes III, X, and V. The right end of III is fused to the left end of X, and the right end of X is fused to the left end of IV. Constructed by crossing eT5 and mnT12. meT7 homozygotes produce 92% viable progeny. meT7 heterozygotes are Him and produce many dead eggs. |
| CB151 |
unc-3(e151) X. |
Unc. |
| CGC28 |
+/szT1 [lon-2(e678) umnIs17] I; dpy-8(e1321) unc-3(e151)/szT1 X. |
umnIs17 [myo-2p::GFP + NeoR, X: 6745526 (intergenic)] I. Heterozygotes are wild-type GFP+, and segregate wild-type GFP+, DpyUnc non-GFP, dead eggs and GFP+ Lon males. Maintain by picking wild-type GFP+. Derived by insertion of myo-2p::GFP transgene into szT1 balancer in parental strain AF1 using CRISPR/Cas9. |
| CGC35 |
+/szT1 [lon-2(e678) umnIs24] I; dpy-8(e1321) unc-3(e151)/szT1 X. |
umnIs24 [myo-2p::GFP + NeoR, X: 15420938 (intergenic)] I. Heterozygotes are wild-type GFP+, and segregate wild-type GFP+, DpyUnc non-GFP, dead eggs and GFP+ Lon males. Maintain by picking wild-type GFP+. Derived by insertion of myo-2p::GFP transgene into szT1 balancer in parental strain AF1 using CRISPR/Cas9. |
| CGC36 |
mnDp1 [umnIs25] (X;V)/+ V; unc-3(e151) X. |
umnIs25 [myo-2p::GFP + NeoR, X: 15420938 (intergenic)]. Pick wild-type GFP+ to maintain. Segregates lethals: homozygous Dp/Dp are lethal. Derived by insertion of myo-2p::GFP transgene into mnDp1 duplication in parental strain SP219 using CRISPR/Cas9. |
| CGC37 |
unc-3(e151) X; mnDp3 [umnIs26] (X;f). |
umnIs26 [myo-2p::GFP + NeoR, X: 15420938 (intergenic)]. Pick wild-type GFP+ to maintain. Segregates wild-type GFP+ and Unc GFP-. Derived by insertion of myo-2p::GFP transgene into mnDp3 duplication in parental strain SP123 using CRISPR/Cas9. |
| CGC40 |
+/szT1 [lon-2(e678) umnIs29] I; dpy-8(e1321) unc-3(e151)/szT1 X. |
umnIs29 [myo-2p::mKate2 + NeoR, X: 6745526 (intergenic)] I. Heterozygotes are wild-type mKate2+, and segregate wild-type mKate2+, DpyUnc non-GFP, dead eggs and mKate2+ Lon males. Maintain by picking wild-type mKate2+. Derived by insertion of myo-2p::mKate2 transgene into szT1 balancer in parental strain AF1 using CRISPR/Cas9. |
| CGC41 |
+/szT1 [lon-2(e678) umnIs30 umnIs24] I; dpy-8(e1321) unc-3(e151)/szT1 X. |
umnIs30 [myo-2p::mKate2 + NeoR, X: 6745526 (intergenic)] I. umnIs24 [myo-2p::GFP + NeoR, X: 15420938 (intergenic)] I. Carries szT1 balancer with mKate2 tag on left arm and GFP tag on right arm. Heterozygotes are wild-type GFP+ & mKate2+, and segregate wild-type GFP+ & mKate2+, DpyUnc non-GFP & non-mKate2, dead eggs and GFP+ & mKate2+ Lon males. Maintain by picking wild-type with GFP+ & mKate2+. Derived by insertion of myo-2p::mKate transgene into szT1 balancer in parental strain CGC35 using CRISPR/Cas9. |
| CGC42 |
mnDp10 [umnIs31] (X;I); unc-3(e151) X. |
umnIs31 [myo-2p::GFP + NeoR, X: 15420938 (intergenic)] I. Segregates mostly wild-type GFP+ and occasional Unc GFP-. Maintain by picking WT GFP+. Derived by insertion of myo-2p::GFP transgene into mnDp10 duplication in parental strain SP117 using CRISPR/Cas9. |
| CGC46 |
+/szT1 [lon-2(e678)] I; dpy-8(e1321) unc-3(e151)/szT1 [umnIs35] X. |
umnIs35 [myo-2p::GFP + NeoR, I: 6284001 (intergenic)] X. Heterozygotes are wild-type GFP+, and segregate wild-type GFP+, DpyUnc non-GFP, dead eggs and GFP+ Lon males. Maintain by picking wild-type GFP+. Derived by insertion of myo-2p::GFP transgene into szT1 balancer in parental strain AF1 using CRISPR/Cas9. |
| CGC50 |
+/szT1 [lon-2(e678) umnIs39] I; dpy-8(e1321) unc-3(e151)/szT1 [umnIs40] X. |
umnIs39 [myo-2p::mKate2 + NeoR, X: 6745526 (intergenic)] I. umnIs40 [myo-2p::GFP + NeoR, I: 6284001 (intergenic)] X. Heterozygotes are wild-type GFP+ & mKate2+, and segregate wild-type GFP+ & mKate2+, DpyUnc non-GFP & non-mKate2, dead eggs, and GFP+ & mKate2+ Lon males. Maintain by picking wild-type with GFP+ & mKate2+. Derived by simultaneous insertion of independent myo-2p::mKate2 and myo-2p::GFP transgenes into each portion of the szT1 balancer in parental strain AF1 using CRISPR/Cas9. |
| CGC79 |
+/szT1 [lon-2(e678) umnIs61] I; dpy-8(e1321) unc-3(e151)/szT1 X. |
umnIs61 [myo-2p::mKate2 + NeoR, X: 15420938 (intergenic)] I. Heterozygotes are wild-type mKate2+, and segregate wild-type mKate2+, DpyUnc non-mKate2, dead eggs and mKate2+ Lon males. Maintain by picking wild-type mKate2+. Derived by insertion of myo-2p::mKate2 transgene into szT1 balancer in parental strain AF1 using CRISPR/Cas9. |
| CGC97 |
+/szT1 [lon-2(e678)] I; dpy-8(e1321) unc-3(e151)/szT1 [umnIs77] X. |
umnIs77 [myo-2p::mKate2 + NeoR, I: 6284001 (intergenic)] X. Heterozygotes are wild-type mKate2+, and segregate wild-type mKate2+, DpyUnc non-mKate2, dead eggs and mKate2+ Lon males. Maintain by picking wild-type mKate2+. Derived by insertion of myo-2p::mKate2 transgene into szT1 balancer in parental strain AF1 using CRISPR/Cas9. |
| DL7 |
wdIs4 II; unc-3(n3435) X. |
wdIs4 [unc-4p::GFP + dpy-20(+)] II. |
| DR111 |
him-4(e1266) unc-3(e151) X. |
Unc. Segregates males. |
| DR1290 |
dpy-6(e14) unc-3(e151) X. |
Medium sized Dpy which curls its tail. |
| GR1452 |
veIs13 V; let-7(mn112) unc-3(e151) X; mgEx725. |
veIs13 [col-19::GFP + rol-6(su1006)] V. mgEx725 [lin-4::let-7 + ttx-3::RFP]. Pick RFP+ to maintain. mgEx725 rescues lethality of let-7(mn112). Precocious expression of col-19::GFP at the L4 stage. Reference: Hayes GD, Riedel CG, Ruvkun G. 2011. Genes Dev. 2011 Oct 1;25(19):2079-92. |
| GS3754 |
sel-13(ok303) III; arIs51 IV; sel-7(n1253) unc-3(e151) X. |
arIs51[cdh-3::GFP]. sel-13=T04A8.10. Reported strong daf-c; raise at 15 C (personal communication to the CGC). Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| KR926 |
hDf6 dpy-5(e61) unc-13(e450)/szT1 [lon-2(e678)] I; unc-3(e151)/szT1 X. |
This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to Ann Rose. |
| KRA102 |
lin-39(kas1) III; ynIs40 V; unc-3(n3435) X. |
ynIs40 [flp-11p::GFP] V. kas1 is a point mutation in the second intron splice acceptor site, disrupting splicing. Worms are vulvaless and have severe locomotion defects. Reference: Feng W, et al. Elife. 2020 Jan 3;9. pii: e50065. doi: 10.7554/eLife.50065. |
| KRA437 |
unc-3(n3435) X; kasEx147. |
kasEx147 [oig-1p(1.6kb)::GFP::unc-54 3'UTR + myo-2p::GFP]. Pick worms with GFP+ pharynx to maintain array. Unc. 1.6kb cis-regulatory region (-2.6-1.0kb) upstream of oig-1 was fused to GFP with the unc-54 3'UTR. oig-1 uses mulitple cis-regulatory regions to achieve different expression patterns in motor neurons; this construct drives expression specifically in cholinergic motor neurons. Construct was injected into N2 worms. Reference: Feng W, et al. Elife. 2020 Jan 3;9. pii: e50065. doi: 10.7554/eLife.50065. |
| KRA439 |
unc-3(n3435) X; kasEx149. |
kasEx149 [oig-1p(2.6kb_del)::GFP::unc-54 3'UTR + myo-2p::GFP]. Pick worms with GFP+ pharynx to maintain array. Unc. 2.6kb cis-regulatory region with 200bp deletion removing predicted LIN-39 binding site upstream of oig-1 was fused to GFP. Expression of oig-1::GFP in GABAergic motor neurons is been abolished; expression was observed specifically in cholinergic motor neurons of the ventral nerve cord. Reference: Feng W, et al. Elife. 2020 Jan 3;9. pii: e50065. doi: 10.7554/eLife.50065. |
| KRA441 |
unc-3(n3435) X; kasEx151. |
kasEx151 [oig-1p(2.6kb_del)::GFP::unc-54 3'UTR + myo-2p::GFP]. Pick worms with GFP+ pharynx to maintain array. Unc. 2.6kb cis-regulatory region with 300bp deletion removing predicted LIN-39 binding site upstream of oig-1 was fused to GFP. Expression of oig-1::GFP in GABAergic motor neurons is retained, but eliminates ectopic expression in cholinergic motor neurons of the ventral nerve cord. Reference: Feng W, et al. Elife. 2020 Jan 3;9. pii: e50065. doi: 10.7554/eLife.50065. |
| MT10362 |
unc-3(n3366) X. |
|
| MT10480 |
unc-3(n3413) X. |
|
| MT2490 |
unc-3(e151) lin-15B&lin-15A(n765) X. |
Unc. Temperature sensitive Muv. |
| MT2718 |
let-2(n1279) unc-3(e151) X; mnDp26 (X;f). |
n1279 is lethal. Animals which are alive contain the duplication. n1279 pka sup-20. |
| NOA2 |
lin-12(n137n460) III; him-5(e1467) V; unc-3(e151) let-7(mn112) X. |
|
| OH10819 |
unc-3(e151) X; vsIs48; otEx4441. |
otEx4441 [hsp-16.2p::unc-3(cDNA) + ttx-3::mCherry]. vsIs48 [unc-17::GFP]. GFP expressed in all cholinergic neurons. Maintain by picking mCherry(+) animals. Reference: Kratsios P, et al. Nat Neurosci. 2011 Nov 27. doi: 10.1038/nn.2989. |
| OH10850 |
unc-3(e151) X; otEx4432. |
otEx4432 [ace-2p(SL2 fosmid)::GFP + elt-2::DsRed2]. Fosmid-based reporter. Maintain by picking DsRed2(+) animals. Reference: Kratsios P, et al. Nat Neurosci. 2011 Nov 27. doi: 10.1038/nn.2989. |
| OH10851 |
juIs14 IV; unc-3(e151) X; otEx4812. |
otEx4812 [unc-30p(2.6kb promoter)::unc-3(cDNA)::unc-54 3'UTR + myo-2::GFP]. juIs14 [acr-2p::GFP + lin-15(+)]. Maintain by picking myo-2::GFP(+) animals. Reference: Kratsios P, et al. Nat Neurosci. 2011 Nov 27. doi: 10.1038/nn.2989. |
| OH13988 |
ieSi57 II; unc-3(ot837[unc-3::mNeonGreen::AID]) X. |
ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. The endogenous unc-3 locus is tagged with mNeonGreen and AID degron. mNeonGreen expression is seen in the cholinergic motor neurons, command interneurons, and ASI. Reference: Patel T & Hobert O. eLife 2017. |
| PQ425 |
apIs320 II; unc-119(ed3) III; unc-3(e151) let-7(mn112) X. |
apIs320 [let-7::unc-119(+)] II. PQ425 was created by crossing PQ320 into unc-3(e151) let-7(mn112) animals, which do not express precursor or mature let-7. unc-119(ed3) might not be homozygous in this strain. Reference: Zisoulis DG, et al. Nature. 2012;486(7404):541-544. |
| PQ426 |
apIs404 II; unc-119(ed3) III; unc-3(e151) let-7(mn112) X. |
apIs404 [let-7(delta alg-1-binding site)::unc-119(+)] II. PQ426 was created by crossing PQ404 into unc-3(e151) let-7(mn112) animals, which do not express precursor or mature let-7. unc-119(ed3) might not be homozygous in this strain. Reference: Zisoulis DG, et al. Nature. 2012;486(7404):541-544. |
| PR946 |
unc-3(p1001) ace-1(p1000) X. |
Acetylcholine abnormal. Kinky Unc. |
| PS1478 |
unc-3(e151) lin-15B&lin-15A(e1763) X. |
Unc. Muv. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| QP122 |
dpy-3(e27) lon-2(e678) unc-3(e151) X. |
Dpy. Unc. dpy-3 is epistatic to lon-2. |
| RW12259 |
unc-3(st12259[unc-3::TY1::EGFP::3xFLAG]) X. |
CRISPR/Cas9 engineered tagged endogenous locus. |
| RW1385 |
mnDp1 (X;V)/+ V; unc-3(e151) pat-9(st558) X. |
Animals with the duplication are WT and segregate WT, sterile and Pats. Strain can be propagated by chunking. |
| SP115 |
mnDp8 (X;I); unc-3(e151) X. |
WT strain. |
| SP116 |
mnDp9 (X;I); unc-3(e151) X. |
WT strain. |
| SP117 |
mnDp10 (X;I); unc-3(e151) X. |
WT strain. Occassionally Uncs appear. |
| SP122 |
unc-3(e151) X; mnDp2 (X;f). |
Strain throws WT and Uncs. Maintain by picking WT. |
| SP123 |
unc-3(e151) X; mnDp3 (X;f). |
Strain throws WT and Uncs. Maintain by picking WT. |
| SP124 |
unc-3(e151) X; mnDp4 (X;f). |
Strain throws WT and Uncs. Maintain by picking WT. |
| SP125 |
unc-3(e151) X; mnDp5 (X;f). |
FREE DUPLICATION. Strain throws WT and Uncs. Maintain by picking WT. |
| SP14 |
unc-3(e151) unc-7(e139) X. |
|
| SP15 |
unc-9(e101) unc-3(e151) X. |
|
| SP1781 |
ncl-1(e1865) unc-36(e251) III; mnDp89 [ncl-1(+) unc-36(+) unc-3(+)] (III;f). |
Animals with mnDp89 are WT. Animals which have lost mnDp89 are Unc and Ncl. mnDp89 not transmitted by males. mnDp89 formed by fusing mnDp14 and mnDp84. |