Gene Information: him-5

Namehim-5 View on WormBase
Species C. elegans
SequenceD1086.4
Genetic positionV:6.09 +/- 0.018 cM
Genomic positionV: 14090426..14092454

Strains carrying this gene

Strain Genotype Description
HZ113 him-5(e1490) bxIs13 V; nfya-1(bp5) X. bxIs13 [egl-5::GFP + lin-15(+)]. Him. Unc. Ectopic expression of GFP in head neurons.
HZ1683 him-5(e1490) V; atg-2(bp576) X. Him; ~30% male.
HZ1684 atg-3(bp412) IV; him-5(e1490) V. Him; ~30% male.
HZ1686 bnIs1 I; atg-7(bp411) IV; him-5(e1490) V. bnIs1 [pie-1p::GFP::pgl-1 + unc-119(+)] I. Him; ~30% male. Accumulation of GFP::PGL-1 aggregates in somatic cells in embryos.
HZ1687 atg-9(bp564) him-5(e1490) V. Him; ~30% male.
HZ1690 epg-6(bp242) III; him-5(e1490) V. Him; ~30% male.
HZ1691 epg-8(bp251) I; him-5(e1490) V. Him; ~30% male.
HZ455 him-5(e1490) V; bpIs131. bpIs131 [sepa-1p::sepa-1::GFP + unc-76(+)]. Him. SEPA-1::GFP aggregates form in a temporal pattern. Diffuse SEPA-1::GFP is detectible in most cells at the comma stage and greatly diminished by the 2-fold stage. After hatching, cytoplasmic SEPA-1::GFP aggregates were found in a few unidentified cells in the head and tail regions and also in the intestine, especially in the anterior and posterior pairs of intestine cells. Reference: Tian Y, et al. Cell. 2010 Jun 11;141(6):1042-55.
HZ504 him-5(e1490) V; bpIs37. bpIs37 [dcap-1p::dcap-1::DsRed + rol-6(su1006)]. Rollers. Translational DsRed reporter for the P body-specific marker dcap-1, which encodes the C. elegans ortholog of decapping complex component DCAP1. Low levels of expression are homogenously distributed in the cytoplasm at all stages of embryogenesis. Reference: Sun YY, et al. Protein Cell. 2011 Nov;2(11):918-39.
HZ589 him-5(e1490) V; bpIs151. bpIs151 [sqst-1p::sqst-1::GFP + unc-76(+)]. Him. In wild-type embryos, sqst-1::GFP is very weakly expressed and diffusely localized in the cytoplasm. Reference: Tian Y, et al. Cell. 2010 Jun 11;141(6):1042-55.
HZ66 sop-2(bp186) II; him-5(e1490) bxIs16 V. bxIs16 [cat-2::YFP + tph-1::CFP]. Temperature sensitive: lethal at 25C. Maintain at 20C.
HZ67 him-5(e1490) bxIs16 V; sor-3(bp185) X. bxIs16 [cat-2::YFP + tph-1::CFP]. Temperature sensitive: lethal at 25C. Maintain at 20C.
HZ769 him-5(e1490) V; bpIs88. bpIs88 [tia-1p::tia-1::GFP + dcap-1p::dcap-1::RFP + rol-6(su1006)]. Rollers. Rolling phenotype is more apparent when raised >20C. Reference: Sun YY, et al. Protein Cell. 2011 Nov;2(11):918-39.
HZ771 him-5(e1490) V; bpIs90. bpIs90 [tia-1p::tia-1::GFP + rol-6(su1006)]. Rollers. Rolling phenotype is more apparent when raised >20C. TIA-1::GFP is homogenously distributed in the cytoplasm during embryogenesis. At larval stages, diffuse TIA-1::GFP signals are observed in seam cells and hypodermal cells. TIA-1::GFP forms distinct bodies, especially in seam cells and hypodermal cells, under stress conditions. Reference: Sun YY, et al. Protein Cell. 2011 Nov;2(11):918-39.
IC400 zdIs5 I; quIs5 II; him-5(e1490) V. zdIs5 [mec-4::GFP + lin-15(+)]. quIs5 [mec-4::myr::vab-1 + rol-6(su1006)]. Animals contain GFP expressed in the touch neurons.
JJ1057 pop-1(zu189) dpy-5(e61)/hT1 I; him-5(e1490)/hT1 V. Heterozygotes are WT and segregate WT, Dpys which give only dead eggs, mid-larval lethals (hT1 homozygotes), males and dead eggs. Mutation in pop-1 results in the MS blastomere adopting the fate of the E blastomere. pop-1 mutant embryos have twice the amount of wild type gut and only make anterior pharynx. him-5 is outside the region balanced by hT2.
JK2505 cyd-1(q626) II; him-5(e1490) V. Temperature-sensitive allele of cyd-1. Phenotypically wild-type at 15C. At 25C, approximately one-third of q626 hermaphrodites were missing one distal tip cell (DTC) and approximately one-half of q626 males were missing the linker cell (LC). q626 also feminizes the XO gonad. q626 affects the production of SGP daughters in both sexes. There is also a maternal component since the mutant phenotype is almost fully penetrant in offspring of homozygous mothers, but less penetrant in offspring of heterozygous mothers. Reference: Tilmann C & Kimble J. Dev Cell. 2005 Oct;9(4):489-99.
JK3375 gld-3(q730)/mIn1 [mIs14 dpy-10(e128)] II; him-5(e1490) V. Heterozygotes are WT with major GFP signal in pharynx. Segregates WT GFP+, Dpy GFP+ (mIn1 homozygotes) and GFP- gld-3 homozygotes. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
JK3437 him-5(e1490) V; qIs74. qIs74 [sys-1p::GFP::pop-1 + unc-119(+)]. Probably on LG X. May have unc-119 in background. Throws 30% males. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
JK590 glp-1(q35)/eT1 III; him-5(e1490)/eT1 V. Heterozygotes are wild-type and segregate wild-type heterozygotes, glp-1(q35) homozygotes (Muv steriles), eT1 homozygotes (Unc-36), and males. glp-1(q35) has a semi-dominant multi-vulva phenotype as well as the loss-of-function Glp phenotype (sterility and embryonic lethality). The q35 mutation is a nonsense mutation that eliminates 122 C-terminal amino acids including a PEST sequence. The C terminus was thought to contain a negative regulatory domain that inactivates glp-1 in the VPCs; the inappropriate glp-1(q35) activity can substitute for lin-12 vulval fate determination. References: Austin J & Kimble J. Cell. 1987 Nov 20;51(4):589-99. Mango S, et al. Nature. 1991 Aug 29;352(6338):811-5.
JT8132 lin-49(sa470) IV; him-5(e1490) V. Males are abnormal. Variable additional defects: Con, Egl, Unc. Temperature sensitive. See also WBPaper00003938.
JT8193 lin-59(sa489) I; him-5(e1490) V. Males are abnormal. Variable additional defects: Con, Egl, Unc. Temperature sensitive. See also WBPaper00003938.
JT9461 lin-48(sa469) III; him-5(e1490) V. Male spicule defect.
KC565 sin-3(tm1276) I; him-5(e1490) V. Mab. Pvl. Throws males.
KS411 lin-17(n671) I; unc-119(e2498) III; him-5(e1490) V; mhIs9. mhIs9 [lin-17::GFP]. Full length lin-17, expressed T.p cells. Rescues lin-17 mutants. unc-119(e2498) may no longer be in the background.
KWN117 pha-1(e2123) III; him-5(e1490) V; rnyEx60. rnyEx60 [vha-6p:::vha-6::mCherry + myo-3p::GFP + pha-1(+)]. Maintain at 25C to select for animals carrying the array. Labels apical intestinal membrane. Reference: Allman et al. (2009) Am J Physiol Cell 297:C1071-81.
KWN190 pha-1(e2123) III; him-5(e1490) V; rnyEx109. rnyEx109 [nhx-2p::D3cpv + pha-1(+)]. Maintain at 20-25C to select for array. KWN190 strain expresses the FRET-based calcium indicator protein D3cpv throughout the cytoplasm of intestinal cells. Dual emission ratio imaging (ex. 435, em. 480/535-nm) can be used to measure intestinal calcium and, although the FRET pair is CFP/YFP, intestinal D3cpv fluorescence is observable under standard GFP filter sets. The D3cpv biosensor has the advantages of being relatively pH-insensitive and not interfering with endogenous calmodulin signaling. References: Am J Physiol Cell Physiol. 301:C1389-1403. Am J Physiol Cell Physiol. 297:C1071-81. FASEB J. 27:760-768. PLoS Biol. 11: e1001613.
LW257 fozi-1(cc609) cup-5(ar465) III; him-5(e1467) V. Missing M-derived coelomocytes and 1-3 body wall muscles. Cells transformed to sex myoblast-like fates.
MH1157 him-5(e1490) V; egl-13(ku194) X. ku194 is a loss of function allele, likely to be molecular null. Connection of gonad defective, >95% Egl. Anchor cell and uterine seam cell do not fuse. Males can mate. Hermaphrodites are very difficult to mate. Previously called cog-2(ku194).
MH1946 dli-1(ku266)/+ IV; him-5(e1490) V. Heterozygotes are WT and segregate WT and Sterile animals with a protruding vulva. Throws males. Clone several WT to recover heterozygote. Cytoplasmic dynein light intermediate chain. 9/02: ku266 is not an L to stop; it is a W117 to a stop (TGG to TGA). Sandhya Koushika.
ML743 rdy-2(mc40)/sqt-3(sc63) him-5(e1467) unc-76(e911) V. Heterozygotes are WT and segregate WT, Rol Uncs, and dead L2 larvae that are translucent and often found away from the bacterial lawn (can be difficult to spot on the lawn).
MT1329 lin-12(n302) III; him-5(e1467) V. Vulvaless. Segregates males which mate well.
MT1373 lin-13(n387)/unc-32(e189) III; him-5(e1467) V. Hets are WT. At 25C hets segregate WT, Unc, Sterile Muv and males. Maintain by picking WT. ts maternal effect.
MT1965 lin-12(n941)/eT1 III; him-5(e1467)/eT1 [him-5(e1467)] V. n941 is a lin-12 null allele. e1467 is also carried on eT1.
MT2021 lin-12(n952) III; him-5(e1467) V. Muv. Throws males.
MT2537 dpy-13(e184) unc-8(n491) IV; him-5(e1490) V. DpyUnc. e184 and n491 are both semi-dominant.
MT2663 sqt-3(sc63) him-5(e1467) egl-1(n986) unc-76(e911) V. Dominant Egl. Unc. Dpy (ts). Throws males.
MT2845 egl-5(n945) III; him-5(e1467) egl-1(n986) V. n945: HSN- . Egl. Coiler.
MT4051 lin-44(n1792) I; him-5(e1490) V. At 20C about 50% of the hermaphrodites are Egl. Males have an abnormal tail and do not mate.
MT4150 unc-17(e245) dpy-4(e1166) IV; him-5(e1490) V. DpyUnc. Segregates males.
MT4156 lin-26(n156)/mnC1 [dpy-10(e128) unc-52(e444)] II; him-5(e1490) V. Heterozygotes are WT and segregate WT, Egls with abnormal tails and DpyUncs.
MT4700 let-60(n1531) unc-22(e66)/let-60(n1700) dpy-20(e1282) IV; him-5(e1490) V. Heterozygotes are mostly non-Muv (about 20% will be Muv because n1700 is semi-dominant) and non-Unc. Hets segregate Twitchers which die at various larval stages or as Vul adults, because of the maternal effect rescue of n1531 by n1700. The Twitchers will not give viable progeny. Hets also segregate DpyMuvs. The strain segregates males which will mate, though not very well.
MT4705 lin-17(n671) lin-44(n1792) I; him-5(e1490) V.
MT4718 egl-31(n472) I; him-5(e1490) V. Egg laying defective. Makes bags of worms. Backward Unc. Throws males.
MT491 him-5(e1467) V; lin-2(e1309) X. Vulvaless. Segregates males.
MT529 him-5(e1467) V; lin-14(n179) X. Abnormal vulva (temperature sensitive). Segregates males.
MT5726 dpy-20(e1362) unc-22(e66) IV; him-5(e1490) V; nDp5 (IV;f). Animals with the Duplication are WT. Animals which have lost the Duplication are Dpy and Twitchers. Throws both WT and DpyTwitcher males. Maintain by picking WT.
MT5823 lin-26(n156) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II; him-5(e1490) V. Heterozygotes are WT.
MT633 lin-11(n389) I; him-5(e1467) V. Vulvaless. Slightly Unc. Throws males.
MT688 lin-12(n137)/unc-32(e189) III; him-5(e1467) V. Heterozygotes are Muv and segregate Muv, Unc and males. Maintain by picking Muv which throw Uncs.