Gene Information: him-5

Namehim-5 View on WormBase
Species C. elegans
SequenceD1086.4
Genetic positionV:6.09 +/- 0.018 cM
Genomic positionV: 14090426..14092454

Strains carrying this gene

Strain Genotype Description
EM122 stDp2 (X;II)/+ II; him-5(e1490) V; unc-18(e81) dpy-6(e14) X. Strain throws WT, DpyUncs and males (and some Lon-don't know where these come from??). Maintain by picking WT. non-Unc non-Dpy males mate at low frequency and will transmit stDp2.
EM128 mab-21(bx53) III; him-5(e1490) V. Transformation of ray 6 to a thin ray which is anteriorly displaced and fuses with ray 4 (95%). A 10th ray is found in about 50% of the sides scored. Slightly shorter than WT. mab-21(bx53)/yDf10 is embryonically lethal with embryo arrest at 2 fold stage.
EM131 ram-2(bx39) II; him-5(e1490) V. Lumpy rays.
EM139 ram-1(bx34) I; him-5(e1490) V. Lumpy rays.
EM140 dpy-5(e61) ram-1(bx34) I; him-5(e1490) V. Dpy. Rays abnormal.
EM141 unc-17(e113) col-34(bx25) IV; him-5(e1490) V. Unc. Abnormal rays. bx25 previously called ram-4.
EM146 dpy-10(e128) ram-2(bx39) II; him-5(e1490) V. Dpy. Abnormal rays.
EM172 unc-4(e120) II; him-5(e1490) V; lin-32(bx46) X. Missing Rays. Unc.
EM207 tbx-2(bx59) III; him-5(e1490) V. Conditional lethal: inviable when grown at 25C. Wild type at 16C. Viable when grown at 20C; partial ray loss at 20C. Shifting males during ray assembly to non-permissive temperature results in ray loss. No lineage defect.
EM253 mab-20(bx61) I; him-5(e1490) V. Ray 3 and 4 fusion >60% at non-permissive temp. Ray 1 and 2 fusion about 10% at non-permissive temp. ts period is around L3-L4.
EM305 efn-4(bx80) IV; him-5(e1490) V. Extensive ray fusion involving all 9 rays. Larva have Vab phenotype with decreasing expressivity in adult. Hermaphrodites have swollen tail and anus. bx80 pka mab-26(bx80).
EM331 him-5(e1490) V; mab-19(bx83) X. Male phenotype: loss of rays 7-9; incomplete penetrance/expressivity (80%); T lineage defect. Hermaphrodite phenotype: lowered brood size (100 progeny/hermaphrodite). Hypomorphic allele: uDf1/mab-19(bx83) results in embryonic arrest during morphogenesis.
EM347 tlp-1(bx85) IV; him-5(e1490) V. Although hermaphrodites appear WT in other ways, there are some problems with T cell lineages (affecting the phasmids) and tail cell fusions. Variably Dyf. Male tail tip morphogenesis is also defective, resulting in blobby, "leptoderan" tails. Males are infertile due to an inability to properly copulate. tlp-1 encodes a nuclear protein with a single C2H2-type zinc finger domain and an N-terminal "SPLALLA" domain, similar to that of Sp1 transcription factors of vertebrates. The bx85 mutation involves a truncation of TLP-1 due to a frameshift caused by a 5-bp deletion.
EM489 pal-1(e2091) III; him-5(e1490) V; dpy-22(bx92) X. V6 produces rays instead of alae in pal-1; sop01 mutants. No obvious phenotype in a pal-1(+) background. Previously called sop-1(bx92).
EM490 pal-1(e2091) III; him-5(e1490) V; dpy-22(bx93) X. V6 produces rays instead of alae in pal-1; sop01 mutants. No obvious phenotype in a pal-1(+) background. Previously called sop-1(bx93).
EM493 sop-3(bx96) I; pal-1(e2091) III; him-5(e1490) V. V6 produces rays instead of alae in sop-3; pal-1 mutants.
EM496 hlh-2(bx108) I; him-5(e1490) V; lin-32(e1926) X. Males are rayless. hlh-2(bx108) strongly and semi-dominantly enhances the ray-loss phenotype of lin-32(e1926).
EM507 pal-1(e2091) III; him-5(e1490) V; dpy-22(bx103) X. V6 produces rays instead of alae in pal-1; sop-1 mutants. No obvious phenotype in a pal-1(+) background. Previously called sop-1(bx103).
EM511 pal-1(e2091) III; him-5(e1490) V; dpy-22(bx107) X. V6 produces rays instead of alae in pal-1; sop01 mutants. No obvious phenotype in a pal-1(+) background. Previously called sop-1(bx107).
EM598 hlh-2(bx115) unc-13(e51) I; him-5(e1490) V. Paralyzed Unc. Throws males. hlh-2(bx115) has no phenotype in this background.
EM599 him-5(e1490) V; lin-15B&lin-15A(n765) X; bxIs13. bxIs13 [egl-5::GFP + lin-15(+)]. Him. egl-5::GFP reporter made from EM#286 (GFP inserted within the last few amino acids of the C-terminus) by integrating bxEx30 in EM588. High nuclear expression of transgene in males (good in seam cells, none in rectal epithelium), weak expression in hermaphrodites. Expression in 2-4 head neurons in males and hermaphrodites. egl-5 gene on reporter does not rescue egl-5(-).
EM62 mab-17(e2167) unc-15(e73) I; him-5(e1490) V. Unc. Round bulging adult male tale. Very reduced fan. Mating efficiency is zero.
EM66 him-5(e1490) V; vab-3(bx23) X. Transformation of ray 6 to a thin ray which is anteriorly displaced and fuses with ray 4 (99%). Body is slightly shorter. See also WBPaper00002235.
EM67 mab-20(bx24) I; him-5(e1490) V. Extensive ray fusion. Posterior portion of body swollen at larval stages.
EM68 col-34(bx25) IV; him-5(e1490) V. Lumpy rays. Temperature sensitive. bx25 previously called ram-4.
EM723 sop-2(bx91) II; him-5(e1490) V. Temperature sensitive. Early larval arrest at 25C. At 20C, Lon, PVul, Muv, Mog, ectopic Hox gene expression.
EM81 him-5(e1490) V; ram-5(bx30) X. Lumpy rays.
EM886 rax-2(bx131) III; bxIs14 him-5(e1490) V. bxIs14 [pkd-2::GFP + pdx-1]. Ray axon defective.
EM902 madd-2(bx132) bxIs14 him-5(e1490) V. bxIs14 [pkd-2::GFP + pdx-1]. Ray axon defective.
EM905 rax-5(bs137) II; bxIs14 him-5(e1490) V. bxIs14 [pkd-2::GFP + pdx-1]. Ray axon defective.
EM907 rax-3(bx133) I; bxIs14 him-5(e1490) V. bxIs14 [pkd-2::GFP + pdx-1]. Ray axon defective.
EM908 rax-3(bx138) I; bxIs14 him-5(e1490) V. bxIs14 [pkd-2::GFP + pdx-1]. Ray axon defective.
EM909 rax-4(bx139) I; bxIs14 him-5(e1490) V. bxIs14 [pkd-2::GFP + pdx-1]. Ray axon defective.
EM911 rax-6(bx140) IV; bxIs14 him-5(e1490) V. bxIs14 [pkd-2::GFP + pdx-1]. Ray axon defective.
EM938 pdfr-1(bx142) III; him-5(e1490) V. Male leaving assay defective (Las), lethargic, hypereversal. Reference: Barrios, A, et al. Nat Neurosci. 2012 Dec;15(12):1675-82.
EM97 ram-2(bx32) II; him-5(e1490) V. Lumpy rays.
EU1472 let-99(or204) IV; him-5(e1490) V. let-99(or204) is temperature-sensitive, maternal-effect, embryonic-lethal. Nearly completely penetrance of lethality at 26C. Viable at 15C. Maintain at 15C. Reference: Goulding MB, et al. J Cell Biol. 2007 Sep 24;178(7):1177-91.
EW61 dpy-20(e1282) IV; him-5(e1490) V; deIs4. deIs4 [ajm-1::GFP + GFP::lin-39(YAC) + dpy-20(+)]. A transcriptional fusion of GFP to the ATG of lin-39, with a stop codon between GFP and LIN-39 sequences. Present on a YAC. Yeast chromosomal DNA was injected and integrated. Expression is very faint and may get fainter over time. Freeze upon receipt.
EW62 smg-1(e1228) I; dpy-20(e1282) IV; him-5(e1490) V; deIs4. deIs4 [ajm-1::GFP + GFP::lin-39(YAC) + dpy-20(+)]. A transcriptional fusion of GFP to the ATG of lin-39, with a stop codon between GFP and LIN-39 sequences. Present on a YAC. Yeast chromosomal DNA was injected and integrated. Expression is very faint and may get fainter over time. Therefore, freeze upon receipt.
GC771 him-5(e1490) V; naEx57. naEx57 [lag-2p::FLP::let-858 3'UTR + ceh-22p::GFP]. Him. Derived by injection of plasmids pGC158 and pCW2.19. Reference: Voutov, R and Hubbard, EJ. Genetics 180(1):103-19 (2008).
GE1709 vab-9(e1744) unc-4(e120) II; him-5(e1490) V. Unc. Slightly Dpy. Tail whip knobbed at all stages except adult male (adult male tail tip slightly swollen). Variably Egl.
GS419 evl-3(ar118)/dpy-10(e128) unc-4(e120) II; him-5(e1467) V. Heterozygotes are WT and segregate WT, DpyUncs and Steriles which have an everted vulva. Throws males. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
HS144 msi-1(os1) III; him-5(e1490) V. 1.6 kb deletion in msi-1. Males have poor mating efficiency.
HS321 him-5(e1467) unc-76(e911) V; osEx67. osEx67 [psa-4::GFP + unc-76(+)]. Maintain by picking non-Unc. Reference: Sawa H, et al. Mol Cell. 2000 Sep;6(3):617-24.
HS325 him-5(e1467) unc-76(e911) V; osEx71. osEx71 [psa-1::GFP + unc-76(+)]. Maintain by picking non-Unc. Reference: Sawa H, et al. Mol Cell. 2000 Sep;6(3):617-24.
HY618 apc-1(or374) II; him-5(e1490) V. Temperature-sensitive embryonic lethal, maintain at 15C. Shift L4 hermaphrodites to 25C overnight for mutant embryos. Mutant embryos are sensitive to osmolarity and pressure. Previously called pod-3 and mat-2..
HY619 apc-1(or385) II; him-5(e1490) V. Temperature-sensitive embryonic lethal, maintain at 15C. Shift L4 hermaphrodites to 25C overnight for mutant embryos. Mutant embryos are sensitive to osmolarity and pressure. Previously called pod-3 and mat-2.
HZ107 him-5(e1490) bxIs13 V; nfya-1(bp4) X. bxIs13 [egl-5::GFP + lin-15(+)]. Him. Unc. Ectopic expression of GFP in head neurons.
HZ111 muIs16 II; sor-1(bp_1)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-5(e1490) V. muIs16 [mab-5::GFP]. Larval lethal at L3/L4 stage. Ectopic expression of GFP in the arrested sor-1 mutant homozygotes (L3 stage). NOTE: This allele is published as bp1, but has been curated in WormBase as bp_1 in order to differentiate it from the STS marker bP1.
HZ112 muIs16 II; sor-1(bp3)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-5(e1490) V. muIs16 [mab-5::GFP]. Larval lethal at L3/L4 stage. Ectopic expression of GFP in the arrested sor-1 mutant homozygotes (L3 stage).