| AD200 |
unc-119(ed3) III; asIs1. |
asIs1 [pie-1p::GFP::egg-3 + unc-119(+)]. |
| AD238 |
asIs2. |
asIs2 [pie-1p::mCherry::egg-3]. |
| EGD175 |
pie-1(ne4301[pie-1::gfp]) III; mex-5(egx1[F294N & F339N]) IV. |
pie-1(ne4301) inserted GFP into pie-1 locus tagging endogenous pie-1 with GFP. mex-5(egx1[F294N, F339N]) modifies the endogenous mex-5 locus to disrupt zinc finger motifs. Reference: Han et al, Current Biology 2017. |
| ET113 |
unc-119(ed3) III; ekIs2. |
ekIs2 contains [pie-1p::GFP::cyb-1 + unc-119(+)]. Translational fusion of CYB-1 expressed from the pie-1 promoter and including the pie-1 3'UTR. GFP::CYB-1 expression in the proximal gonad, with staining disappearing in the zygote. Maintain at 25°C. |
| EU16 |
dpy-18(e364) pie-1(zu154) III; skn-1(zu67)/nT1 [unc-?(n754) let-?] IV; +/nT1 V; eDp6 (III;f). |
Heterozygotes are Unc. Segregates Uncs (dpy-18 pie-1; skn-1/DnT1; eDp6), Dpy non-Uncs (dpy-18 pie-1; skn-1 with no Dp) which give only dead embryos, and DpyUncs (dpy-18 pie-1; skn-1/DnT1 with no Dp) which give only dead eggs, WT looking (dpy-18 pie-1; skn-1; eDp6) which give only dead embryos, and dead eggs. |
| EU3201 |
klp-15(ok1958) aspm-1(syb1260[gfp::aspm-1]) klp-16(or1952) /tmC18[dpy-5(tmIs1236)] I; ltIs37[pie-1p::mCherry::H2B::pie-1 3'UTR + unc-119(+)] IV |
ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. ruIs57 [pie-1p::GFP::tubulin + unc-119(+)]. GFP tag inserted into endogenous aspm-1 locus. tmC18 balancer marked with myo-2p::mCherry and Dpy. Heterozygotes are wild-type with pharyngeal mCherry, and segregate mCherry+ heterozygotes, tmC18 homozygotes (mCherry+ Dpy) and non-mCherry triple mutant homozygotes. Homozygous triple mutants are fertile but produced reduced brood sizes with highly penetrant embryonic lethality; will also segregate some males. [NOTE: the ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV transgene was previously annotated as itIs37 in this strain. The correct name of the transgene is ltIs37 and not itIs37.] Reference: Chuang CH, et al., Biology Open 2020 9: bio052308 doi: 10.1242/bio.052308 Published 25 June 2020 |
| JA1334 |
unc-119(e2498) III; weIs11. |
weIs11[unc-119(+) + TAC-1::GFP]. |
| JH1327 |
axEx73. |
axEx73 [pie-1p::pie-1::GFP + rol-6(su1006) + N2 genomic DNA]. pie-1::GFP is expressed in embryos and oocytes. This array may have integrated spontaneously since the strain segregates 100% Rollers. |
| JH1448 |
axEx1125. |
axEx1125 [pie-1p::mex-5::GFP::pie-1 3’UTR + rol-6(su1006) + N2 genomic DNA]. Maintain at 25C. Pick Rollers to maintain. axEx1125 contains pKR2.04, a construct carrying MEX-5::GFP in vector pKR1.42; pKR1.42 uses the pie-1 promoter, enhancer, and 3′ UTR to drive maternal expression of GFP in embryos. Animals with the array are Rollers. Animals which have lost the array are WT. |
| JH1473 |
itIs153; ojIs1. |
itIs153 [pie-1p::par-2::GFP + rol-6(su1006) + N2 genomic DNA]. ojIs1 [pie-1p::GFP::tbb-2 + unc-119(+)]. Maintain at 25C. Constructed by crossing heterozygous ojIs1 males into KK866 (itIs153) hermaphrodites and selecting for double homozygosity of the arrays. itIs153 is an integrated derivitive of axEx1094. |
| JJ532 |
pie-1(zu154) unc-25(e156)/qC1 [dpy-19(e1259) glp-1(q339)] III. |
Heterozygotes are WT and segregate WT, Unc and DpySteriles. Uncs give only dead eggs. Maintain by picking WT. |
| KK866 |
itIs153. |
itIs153 [pie-1p::par-2::GFP + rol-6(su1006) + N2 genomic DNA]. Maintain at 25C. itIs153 is an integrated derivitive of axEx1094. |
| NL3630 |
pkIs32 III; eri-1(mg366) IV. |
pkIs32[pie-1::GFP::H2B]. RNAi hypersensitive. |
| NL3847 |
pkIs1600 I; ruIs32 III. |
pkIs1600 [dpy-30::GFP(truncated) + rol-6(su1006)] I. ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. Rollers. |
| OC95 |
bsIs2 ruIs32 III. |
bsIs2 [pie-1::GFP::spd-2 + unc-119(+)]. ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. Wild type animals express GFP marked DNA (GFP-H2b) and centrosomes (GFP-SPD-2) in germ line and embyros. Maintain at 24C to obtain optimal GFP expression. |
| OD176 |
unc-119(ed3) III; ltIs103. |
ltIs103 [(pAA212) pie-1p::GFPLAP::cav-1 + unc-119(+)]. |
| OD177 |
unc-119(ed3) III; ltIs104. |
ltIs104 [(pAA277) pie-1p::GFP::LAP::vps-37 + unc-119(+)]. |
| OD178 |
unc-119(ed3) III; ltIs105. |
ltIs105 [(pAA280) pie-1p::GFP::LAP::MVB-12 + unc-119(+)]. |
| OD179 |
unc-119(ed3) III; ltIs79; pwIs116. |
ltIs79 [(pAA196) pie-1p::mCherry::rab-5 + unc-119(+)]. pwIs116 [rme-2p::rme-2::GFP::rme-2 3'UTR + unc-119(+)]. Maintain at 20-25C to reduce silencing of the array. |
| OD58 |
unc-119(ed3) III; ltIs38. |
ltIs38 [pie-1p::GFP::PH(PLC1delta1) + unc-119(+)]. |
| OD61 |
unc-119(ed3) III; ltIs41. |
ltIs41[pAA5; pie-1::GFP-TEV-STag::CAR-1; unc-119(+)]. |
| OD62 |
unc-119(ed3) III; ltIs42. |
ltIs42[pAA19; pie-1::GFP-TEV-Stag::CAR-1deltaN; unc-119(+)]. |
| OD63 |
unc-119(ed3) III; ltIs43/+. |
ltIs43[pAA26; pie-1::GFP-TEV-STag::ZEN-4; unc-119(+)]. Insertion only viable when heterozygous. Pick non-Unc worms to maintain. |
| OD76 |
unc-119(ed3) III; ltIs75. |
ltIs75 [(pSK5) pie-1::GFP::TEV-STag::LacI + unc-119(+)]. |
| OD83 |
unc-119(ed3) III; ltIs37 IV; qaIs3507. |
ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. qaIs3507 [pie-1::GFP::lem-2 + unc-119(+)]. |
| RW10006 |
unc-119(ed3) ruIs32 III; zuIs178 V. |
ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. zuIs178 [his-72(1kb 5' UTR)::his-72::SRPVAT::GFP::his-72 (1KB 3' UTR) + 5.7 kb XbaI - HindIII unc-119(+)]. Ubiquitous histone-GFP fusion protein in embryo and adult germline as well as many adult somatic cells. |
| SS747 |
bnIs1. |
bnIs1[pie-1::GFP::pgl-1 + unc-119(+)]. GFP is brightest at 25C and the strain should be grown at that temperature. Routinely pick bright GFP+ worms to maintain. Because the transgene can become silenced, you should check the worms for GFP expression and freeze as soon as possible. |
| SV2061 |
he314[pie-1p::GLO-ePDZ::mCherry::smu-1::tbb-2 3'UTR] II; e259[eft-3p::PH::eGFP::LOV::tbb-2 3'UTR]) IV. |
he314[pie-1p::GLO-ePDZ::mCherry::smu-1::tbb-2 3'UTR] II. e259[eft-3p::PH::eGFP::LOV::tbb-2 3'UTR]) IV. Superficially wild-type. CRISPR/Cas9 was used to create insertion alleles he314 and he259 insertions into N2 background at sites of known MosSCI insertions ttTi5605 and cxTi10816, respectively. ePDZ–LOV system transgenes allow use of blue light to control protein heterodimerization, in this case, membrane recruitment of ePDZ-tagged proteins of interest. Germline-optimized cytosolic ePDZ::mCherry-tagged SMU-1 (GLO-ePDZ::mCherry::SMU-1), and membrane-bound LOV2 domain fused to a pleckstrin-homology domain (PH::eGFP::LOV). GLO-ePDZ::mCherry is a germline-optimized variant coded to be less prone to silencing in the germline. Reference: Fielmich LE, et al. eLife 2018 Aug 15;7:e38198. doi: 10.7554/eLife.38198. |
| TH26 |
unc-119(ed3) III; ddEx10. |
ddEx10 [pie-1p::GFP::sas-4 + unc-119(+)]. Maintain by picking non-Unc. |
| TH27 |
unc-119(ed3) III; ddIs6 V. |
ddIs6 [tbg-1::GFP + unc-119(+)] V. |
| TH32 |
unc-119(ed3) ruIs32 III; ddIs6 V. |
ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. ddIs6 [tbg-1::GFP + unc-119(+)] V. |
| TX773 |
teIs65 II; unc-119(ed3) III; him-3(e1147) IV. |
teIs65 [pie-1p::GFP::plk-1(PBD) + unc-119(+)] II. Maintain at 25 degrees and by picking the most brightly fluorescing animals to avoid silencing of the transgene. teIs65 contains GFP fused to the PLK-1 protein-binding domain. Derived from injection of pRL1216. Reference: Nishi Y, et al. Development. 2008 Feb;135(4):687-97. |
| TX964 |
unc-119(ed3) III; him-3(e1147) IV; teIs98. |
teIs98 [(pRL1450) pie-1p::GFP::sys-1 + unc-119(+)]. |
| WH204 |
unc-119(ed3) III; ojIs1. |
ojIs1 [pie-1p::GFP::tbb-2 + unc-119(+)]. Maintain under normal conditions. Reference: Strome et al. (2001) Mol Biol Cell (6):1751-64. |
| WH327 |
unc-119(ed3) III; ojIs23. |
ojIs23 [pie-1p::GFP::C34B2.10]. |
| WH351 |
unc-119(ed3) III; ojIs37. |
ojIs37 [pie-1p::GFP::ugtp-1 + unc-119(+)]. Maintain under normal conditions. Reference: Bembenek J, et al. Development. (2007) 134(21):3837-48. |