| AA278 |
dhIs59. |
dhIs59 [Topo::daf-9::GFP + lin-15(+)]. Perinuclear expression in a ventral pair of bilateral neurons identified as the IL1Vs or URAVs in the anterior ganglia. By mid-L2, expression in the cytoplasm of the hypodermis, the syncitial epidermis, but absent from midline, epidermal seam cells. Levels peak around the L2 molt and diminish during L4. In some cases, transient expression seen in the L3 vulval blast cells. Also expressed within the hermaphrodite spermatheca starting in late L4 larvae and continuing eve in old adults. In males, expression in IL1V/URAVs and hypodermis but not somatic gonad. In dauer larvae, strong expression in IL1V/URAV and specifically extends into axonal but not dendritic processes. In post-dauer stages, expression in a pattern similar to reproductively growing animals, except expression is absent in the hypodermis. Grow at 20C. May still contain lin-15(n765) mutation in the background. |
| CB2620 |
daf-9(e1406)/lon-2(e678) X. |
Heterozygotes are WT and segregate WT, lethal dauers (DAUER-LIKE LARVAE) and Lon. Can recombine; check for correct segregation of progeny to maintain. |
| CF2263 |
daf-16(mu86) I; glp-1(e2141) III; daf-9(rh50) X; muEx158. |
muEx158 [daf-16AM::GFP + sur-5p::GFP]. Pick GFP+ worms to maintain. Sterile at 25C; grow at 20C or less. |
| CF2288 |
daf-16(mu86) I; glp-1(e2141) III; daf-9(rh50) X; muEx248. |
muEx248 [daf-16p::GFP::DAF-16 cDNA + odr-1p::RFP]. Sterile at 25C; grow at 20C or less. Pick RFP+/GFP+ animals to maintain. |
| DR2281 |
daf-9(m540) X. |
Forms dauer larvae non-conditionally but only arrests at dauer stage for 1-2 days, then grows to adult. daf-c but not ts - can grow at 15C or 20C. |
| DR733 |
lon-2(e678) daf-9(e1406)/unc-78(e1217) X. |
Balanced lethal dauer constitutive. Heterozygotes are WT and segregate WT, LonDaf and Unc. Will get 3-5 adult Lon per clone. Maintain by picking WT. |
| DR921 |
dpy-7(sc27) daf-9(e1406)/lon-2(e678) X. |
Heterozygotes are WT. At 25C the hets segregate DpyDafs, WT and Lon. At 15C the hets segregate Dafs, WT and Lon. [dpy-7(sc27) is temperature sensitive. At 25C it is larger than most Dpys and has a tendency to roll. Not expressed at 15C.] Maintain by picking WT. |
| GR2118 |
daf-9(e1406) dpy-7(sc27) X; mgEx663. |
mgEx663 [dpy-7p::daf-9B(cDNA)::GFP + mec-7::GFP]. Pick GFP+ to maintain. GFP fusion containing 0.4 kb dpy-7 promoter and daf-9 isoform B cDNA. Reference: Mak HY, Ruvkun G. Development. 2004 Apr;131(8):1777-86. |
| HGA8012 |
glp-1(e2141) III; daf-9(rh50) X; lynEx1. |
lynEx1 [nhr-80p::nhr-80::GFP + myo-2p::DsRed]. Sterile at 25C. Maintain at 15-20C. Temperature-sensitve Daf-c. Mig. Pick DsRed+ animals to maintain. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599. |
| NJ185 |
daf-9(rh50) X. |
Unreflexed gonad. Previously known as mig-8. |
| RG1228 |
daf-9(rh50) X. |
Hypomorphic allele. Temperature-sensitve Daf-c. Maintain at 15 C. Reference: Gerisch B, Antebi A. Development. 2004 Apr;131(8):1765-76. |
| VC305 |
+/szT1 [lon-2(e678)] I; daf-9(gk160)/szT1 X. |
T13C5.1. Heterozygotes are WT and segregate WT, arrested szT1 aneuploid progeny, Lon-2 males, and homozygous gk160 hermaphrodites (arrested dauer-like larvae). Pick WT hermaphrodites and check for correct segregation of progeny to maintain. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 |
| VC480 |
+/szT1 [lon-2(e678)] I; daf-9(ok751)/szT1 X. |
T13C5.1. Heterozygotes are WT and segregate WT, arrested szT1 aneuploids, and ok751 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. [CGC NOTE: this strain segregates Lon-2 males at a far lower rate than other szT1 strains; we don't know why, but pick individuals and score progeny carefully.] Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 |