| AA1 |
daf-12(rh257) X. |
daf-d. Strong heterochronic phenotypes in seam, somatic gonad, intestine. Class I allele. Occasional abnormal dauers under exhausted conditions. |
| AA10 |
daf-12(rh286) X. |
Weak heterochronic phenotypes in seam, intestine, somatic gonad. Class V allele. |
| AA120 |
dhIs26. |
dhIs26 [daf-12a::GFP + lin-15(+)]. DAF-12::GFP localized primarily in nucleus, except during mitosis. Expressed widely in most cells including tissues modified for dauer formation or by stage from embryo to adult, but most elevated and widespread during L2. |
| AA18 |
daf-12(rh61rh412) X. |
daf-d. Weak heterochronic phenotypes in seam, somatic gonad and intestine. Class III allele. |
| AA34 |
daf-12(rh61) X. |
daf-d. Strong heterochronic phenotypes in seam, somatic gonad, intestine. Class I allele. |
| AA6 |
daf-12(rh84) X. |
daf-d. Strong heterochronic phenotypes in seam, somatic gonad, intestine. Class I allele. |
| AA82 |
daf-12(rh284) X. |
Gonadal lead cell Mig. Weak heterochronic phenotype in intestine. Weakly daf-c at 25C. Class V allele. |
| AA83 |
daf-12(rh62rh157) X. |
daf-d. Strong heterochronic phenotypes in seam and intestine. Weak heterochronic phenotypes in somatic gonad. Class II allele. |
| AA85 |
daf-12(rh285) X. |
Strong heterochronic phenotypes in seam, somatic gonad, and intestine. Weakly daf-c at 15C. Class IV allele. |
| AA86 |
daf-12(rh61rh411) X. |
Daf-d, weak heterochronic phenotypes in seam, somatic gonad, intestine. Class III allele. |
| AA87 |
daf-12(rh273) X. |
Daf-c, gonadal Mig, weak heterochronic phenotypes in intestine and seam. Class VI allele. |
| AA88 |
daf-12(rh193) X. |
Strong heterochronic phenotypes in seam, somatic gonad, and intestine. Heterochronic phenotypes less penetrant at 15C. Weakly daf-c at 25C. Class IV allele. |
| AA89 |
daf-12(rh274) X. |
daf-c. Gonadal Mig. Weak heterochronic phenotypes in intestine. Class VI allele. |
| ALF3 |
unc-119(ed3) III; daf-12(rh61rh411) X. |
Daf-d. Unc. |
| ALF4 |
unc-119(ed3) III; daf-12(rh61rh411) X; bafIs4. |
bafIs4 [daf-12 (fosmid) + unc-119(+)]; rescues both daf-12 and unc-119. Reference: Hochbaum D, et al. PLoS Genet. 2011 Jul;7(7):e1002179. |
| ALF82 |
unc-119(ed3) III; daf-12(rh61rh411) X; bafIs62. |
bafIs62 [lin-42p::GFP + unc-119(+)]. lin-42p::GFP reporter consists of 2 kb upstream of lin-42a isoform subcloned into modified pPD95.75 vector also carrying unc-119(+). Array was crossed into strain ALF3 to create ALF82. Reference: Hochbaum D, et al. PLoS Genet. 2011 Jul;7(7):e1002179. |
| ALF9 |
unc-119(ed3) III; daf-12(rh61rh411) X; bafIs9. |
bafIs9 [daf-12::TAP (fosmid) + unc-119(+)]; rescues both daf-12 and unc-119. TAP tag inserted into daf-12 fosmid. Reference: Hochbaum D, et al. PLoS Genet. 2011 Jul;7(7):e1002179. |
| CF2248 |
daf-16(mu86) I; glp-1(e2141) III; daf-12(rh61rh411) X; muEx158. |
muEx158 [daf-16AM::GFP + sur-5p::GFP]. Pick GFP+ worms to maintain. Sterile at 25C; grow at 20C or less. |
| CF2278 |
daf-16(mu86) I; glp-1(e2141) III; daf-12(rh61rh411) X; muEx248. |
muEx248 [daf-16p::GFP::DAF-16 cDNA + odr-1p::RFP]. Sterile at 25C; grow at 20C or less. Pick RFP+/GFP+ animals to maintain. |
| DR1191 |
daf-12(m20) unc-27(e155) egl-15(n484) X. |
Dauer defective. Egl-60% form bags of worms. Unc-sluggish, poor backing. Resistant to both imipramine and serotonin. Slightly Dpy. |
| DR1407 |
daf-12(m583) X. |
daf-d. Weak heterochronic phenotypes in seam, somatic gonad, intestine. Class III allele. |
| DR20 |
daf-12(m20) X. |
Defective dauer formation. Hermaphrodites accumulate oocytes. Males mate poorly. |
| DR205 |
lon-2(e678) daf-12(m20) X. |
Dauer defective. Long. |
| DR25 |
daf-12(m25) X. |
Dauer defective. Class 2 suppressor of dauer constitutives. Chemotaxis normal. See also WBPaper00002149. [Previously called daf-20.] |
| DR427 |
daf-12(m116) X. |
daf-d. Weak heterochronic phenotypes in seam, somatic gonad, intestine. Class III allele. |
| DR978 |
daf-12(m419) X. |
daf-d. Weak heterochronic phenotypes in seam, somatic gonad, and intestine. Class III allele. |
| DR979 |
daf-12(m420) X. |
daf-d. Weak heterochronic phenotypes in intestine. Class III allele. |
| DR980 |
daf-12(m421) X. |
daf-d. Weak heterochronic phenotypes in seam, somatic gonad, intestine. Class III allele. |
| DR981 |
daf-12(m422) X. |
daf-d. Weak heterochronic phenotypes in seam, somatic gonad, intestine. Class III allele. |
| DR983 |
daf-12(m424) X. |
daf-d. Weak heterochronic phenotypes in intestine. Class III allele. |
| HGA8005 |
glp-1(e2141) III; daf-12(rh61rh411) X; lynEx1. |
lynEx1 [(pJG01)nhr-80p::nhr-80::GFP + myo-2p::DsRed]. Sterile at 25C. Maintain at 20C or lower. Pick animals with red pharynx to maintain. Lifespan of animals carrying the array is longer than that of glp-1; daf-12 double mutants. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599. |
| JT156 |
daf-12(sa156) X. |
daf-d. Weak heterochronic phenotypes in seam, somatic gonad, intestine. Class III allele. |
| JT204 |
daf-12(sa204) X. |
Lon. Daf-d. |
| JT6924 |
daf-19(m86) II; daf-12(sa204) X. |
Lon. Daf-d. Suppresses Daf-c phenotype of daf-19(m86). Dyf. |
| MH4810 |
elt-1(ku491) IV; wIs51 V; daf-12(rh61rh411) X; kuEx194. |
wIs51 [SCMp::GFP + unc-119(+)] V. kuEx194 [elt-1(+) + sur-5p::DsRed]. GFP expression in seam cells. Pick DsRed+ animals to maintain. In a daf-12(WT) background, elt-1(ku491) exhibits some precocious fusion of seamcells and gaps in alae. elt-1(ku491); daf-12(rh61rh411) double mutants have more sever heterochronic phenotypes including seamcell proliferation and bursting vulvae. Reference: Cohen ML, et al. PLoS Genet. 2015 Mar 27;11(3):e1005099. |
| NJ207 |
daf-12(rh62) X |
Daf-constitutive allele rh62 forms partial-dauer larvae under replete conditions at all temperatures. |
| NJ227 |
daf-12(rh84) X |
Delayed gonadal and extragonadal development. Hermaphrodites (n=100), 74% |
| OH14589 |
daf-12(ot870[daf-12::GFP::3xFlag]) X. |
Superficially wildtype. GFP tag inserted into endogenous daf-12 locus through CRISPR/Cas9 engineering. Reference: Aghayeva et al., submitted |
| OH14891 |
daf-12(ot874[daf-12::TagRFP::3xFlag::AID]) X. |
Superficially wildtype. CRISPR/Cas9-engineered AID conditional daf-12 allele. Reference: Aghayeva et al., submitted |
| OH14984 |
daf-7(e1372) III; daf-12(ot874[daf-12::TagRFP::AID]) X. |
Temperature sensitive dauer constitutive. Maintain at 15C. CRISPR/Cas9-engineered AID conditional daf-12 allele in daf-7(e1372) background (TIR1-less control). Reference: Aghayeva et al., submitted |
| OH14986 |
ieSi57 II; daf-7(e1372) III; daf-12(ot874[daf-12::TagRFP-T::AID]) X. |
ieSi57 [eft-3p::TIR1::mRuby + unc-119(+)] II. Maintain at 15C. Temperature-sensitive dauer constitutive. CRISPR/Cas9-engineered AID conditional daf-12 allele in daf-7(e1372) background with ubiquitous TIR1 expression. Reference: Aghayeva et al., submitted |
| OH14989 |
ieSi60 II; daf-7(e1372) III; daf-12(ot874[daf-12::TagRFP-T::AID]) X. |
ieSi60 [myo-2p::TIR1::mRuby + unc-119(+)] II. Maintain at 15C. Temperature-sensitive dauer constitutive. Pharyngeal muscle-specific depletion of DAF-12 in the presence of auxin. Reference: Aghayeva et al., submitted |
| OH15913 |
daf-7(e1372) III; daf-12(ot874[daf-12::TagRFP::AID]) X; otIs730. |
otIs730 [UPN::TIR1::mTur2 + inx-6(prom18)::TagRFP]. Temperature sensitive dauer constitutive. Maintain at 15C. UPN (Ultra Pan-Neuronal) promoter contains four short pan-neuronal promoters fused together (unc-11::rgef-1::ehs-1::ric-19). Panneuronal depletion of DAF-12 in the presence of auxin. Reference: Aghayeva et al., submitted |
| VC243 |
daf-12(ok493) X. |
F11A1.3, F11A1.2. Superficially wild type. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 |
| VT3922 |
lin-28(n719) I; daf-12(ma497[daf-12::gfp]) hbl-1(ma430[hbl-1::mScarlet-I]) X. |
Precocious heterochronic phenotypes as preciously reported for lin-28(n719). Endogenous daf-12 locus tagged with GFP. hbl-1(ma430) is a CRISPR/Cas9-edited allele, which contains a linker and the mScarlet-I sequence integrated in-frame with hbl-1. Reference: Ilbay O & Ambros V. Development. 2019 Nov 6;146(21). |
| VT3923 |
maIs105 V; daf-12(ma498[daf-12::mScarlet-I]) X. |
maIs105 [col-19p::GFP] V. mScarlet-I tag inserted at C-terminus of endogenous daf-12 locus through CRISPR/Cas9-engineering. Reference: Ilbay O & Ambros A. Development. 2019 Oct 9. pii: dev.183111. doi: 10.1242/dev.183111. |