Gene Information: daf-12

Namedaf-12 View on WormBase
Species C. elegans
SequenceF11A1.3
Genetic positionX:2.37 +/- 0.000 cM
Genomic positionX: 10635908..10666875

Strains carrying this gene

Strain Genotype Description
AA1 daf-12(rh257) X. daf-d. Strong heterochronic phenotypes in seam, somatic gonad, intestine. Class I allele. Occasional abnormal dauers under exhausted conditions.
AA10 daf-12(rh286) X. Weak heterochronic phenotypes in seam, intestine, somatic gonad. Class V allele.
AA120 dhIs26. dhIs26 [daf-12a::GFP + lin-15(+)]. DAF-12::GFP localized primarily in nucleus, except during mitosis. Expressed widely in most cells including tissues modified for dauer formation or by stage from embryo to adult, but most elevated and widespread during L2.
AA18 daf-12(rh61rh412) X. daf-d. Weak heterochronic phenotypes in seam, somatic gonad and intestine. Class III allele.
AA34 daf-12(rh61) X. daf-d. Strong heterochronic phenotypes in seam, somatic gonad, intestine. Class I allele.
AA6 daf-12(rh84) X. daf-d. Strong heterochronic phenotypes in seam, somatic gonad, intestine. Class I allele.
AA82 daf-12(rh284) X. Gonadal lead cell Mig. Weak heterochronic phenotype in intestine. Weakly daf-c at 25C. Class V allele.
AA83 daf-12(rh62rh157) X. daf-d. Strong heterochronic phenotypes in seam and intestine. Weak heterochronic phenotypes in somatic gonad. Class II allele.
AA85 daf-12(rh285) X. Strong heterochronic phenotypes in seam, somatic gonad, and intestine. Weakly daf-c at 15C. Class IV allele.
AA86 daf-12(rh61rh411) X. Daf-d, weak heterochronic phenotypes in seam, somatic gonad, intestine. Class III allele.
AA87 daf-12(rh273) X. Daf-c, gonadal Mig, weak heterochronic phenotypes in intestine and seam. Class VI allele.
AA88 daf-12(rh193) X. Strong heterochronic phenotypes in seam, somatic gonad, and intestine. Heterochronic phenotypes less penetrant at 15C. Weakly daf-c at 25C. Class IV allele.
AA89 daf-12(rh274) X. daf-c. Gonadal Mig. Weak heterochronic phenotypes in intestine. Class VI allele.
ALF3 unc-119(ed3) III; daf-12(rh61rh411) X. Daf-d. Unc.
ALF4 unc-119(ed3) III; daf-12(rh61rh411) X; bafIs4. bafIs4 [daf-12 (fosmid) + unc-119(+)]; rescues both daf-12 and unc-119. Reference: Hochbaum D, et al. PLoS Genet. 2011 Jul;7(7):e1002179.
ALF82 unc-119(ed3) III; daf-12(rh61rh411) X; bafIs62. bafIs62 [lin-42p::GFP + unc-119(+)]. lin-42p::GFP reporter consists of 2 kb upstream of lin-42a isoform subcloned into modified pPD95.75 vector also carrying unc-119(+). Array was crossed into strain ALF3 to create ALF82. Reference: Hochbaum D, et al. PLoS Genet. 2011 Jul;7(7):e1002179.
ALF9 unc-119(ed3) III; daf-12(rh61rh411) X; bafIs9. bafIs9 [daf-12::TAP (fosmid) + unc-119(+)]; rescues both daf-12 and unc-119. TAP tag inserted into daf-12 fosmid. Reference: Hochbaum D, et al. PLoS Genet. 2011 Jul;7(7):e1002179.
CF2248 daf-16(mu86) I; glp-1(e2141) III; daf-12(rh61rh411) X; muEx158. muEx158 [daf-16AM::GFP + sur-5p::GFP]. Pick GFP+ worms to maintain. Sterile at 25C; grow at 20C or less.
CF2278 daf-16(mu86) I; glp-1(e2141) III; daf-12(rh61rh411) X; muEx248. muEx248 [daf-16p::GFP::DAF-16 cDNA + odr-1p::RFP]. Sterile at 25C; grow at 20C or less. Pick RFP+/GFP+ animals to maintain.
DR1191 daf-12(m20) unc-27(e155) egl-15(n484) X. Dauer defective. Egl-60% form bags of worms. Unc-sluggish, poor backing. Resistant to both imipramine and serotonin. Slightly Dpy.
DR1407 daf-12(m583) X. daf-d. Weak heterochronic phenotypes in seam, somatic gonad, intestine. Class III allele.
DR20 daf-12(m20) X. Defective dauer formation. Hermaphrodites accumulate oocytes. Males mate poorly.
DR205 lon-2(e678) daf-12(m20) X. Dauer defective. Long.
DR25 daf-12(m25) X. Dauer defective. Class 2 suppressor of dauer constitutives. Chemotaxis normal. See also WBPaper00002149. [Previously called daf-20.]
DR427 daf-12(m116) X. daf-d. Weak heterochronic phenotypes in seam, somatic gonad, intestine. Class III allele.
DR978 daf-12(m419) X. daf-d. Weak heterochronic phenotypes in seam, somatic gonad, and intestine. Class III allele.
DR979 daf-12(m420) X. daf-d. Weak heterochronic phenotypes in intestine. Class III allele.
DR980 daf-12(m421) X. daf-d. Weak heterochronic phenotypes in seam, somatic gonad, intestine. Class III allele.
DR981 daf-12(m422) X. daf-d. Weak heterochronic phenotypes in seam, somatic gonad, intestine. Class III allele.
DR983 daf-12(m424) X. daf-d. Weak heterochronic phenotypes in intestine. Class III allele.
HGA8005 glp-1(e2141) III; daf-12(rh61rh411) X; lynEx1. lynEx1 [(pJG01)nhr-80p::nhr-80::GFP + myo-2p::DsRed]. Sterile at 25C. Maintain at 20C or lower. Pick animals with red pharynx to maintain. Lifespan of animals carrying the array is longer than that of glp-1; daf-12 double mutants. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599.
JT156 daf-12(sa156) X. daf-d. Weak heterochronic phenotypes in seam, somatic gonad, intestine. Class III allele.
JT204 daf-12(sa204) X. Lon. Daf-d.
JT6924 daf-19(m86) II; daf-12(sa204) X. Lon. Daf-d. Suppresses Daf-c phenotype of daf-19(m86). Dyf.
MH4810 elt-1(ku491) IV; wIs51 V; daf-12(rh61rh411) X; kuEx194. wIs51 [SCMp::GFP + unc-119(+)] V. kuEx194 [elt-1(+) + sur-5p::DsRed]. GFP expression in seam cells. Pick DsRed+ animals to maintain. In a daf-12(WT) background, elt-1(ku491) exhibits some precocious fusion of seamcells and gaps in alae. elt-1(ku491); daf-12(rh61rh411) double mutants have more sever heterochronic phenotypes including seamcell proliferation and bursting vulvae. Reference: Cohen ML, et al. PLoS Genet. 2015 Mar 27;11(3):e1005099.
NJ207 daf-12(rh62) X Daf-constitutive allele rh62 forms partial-dauer larvae under replete conditions at all temperatures.
NJ227 daf-12(rh84) X Delayed gonadal and extragonadal development. Hermaphrodites (n=100), 74%
OH14589 daf-12(ot870[daf-12::GFP::3xFlag]) X. Superficially wildtype. GFP tag inserted into endogenous daf-12 locus through CRISPR/Cas9 engineering. Reference: Aghayeva et al., submitted
OH14891 daf-12(ot874[daf-12::TagRFP::3xFlag::AID]) X. Superficially wildtype. CRISPR/Cas9-engineered AID conditional daf-12 allele. Reference: Aghayeva et al., submitted
OH14984 daf-7(e1372) III; daf-12(ot874[daf-12::TagRFP::AID]) X. Temperature sensitive dauer constitutive. Maintain at 15C. CRISPR/Cas9-engineered AID conditional daf-12 allele in daf-7(e1372) background (TIR1-less control). Reference: Aghayeva et al., submitted
OH14986 ieSi57 II; daf-7(e1372) III; daf-12(ot874[daf-12::TagRFP-T::AID]) X. ieSi57 [eft-3p::TIR1::mRuby + unc-119(+)] II. Maintain at 15C. Temperature-sensitive dauer constitutive. CRISPR/Cas9-engineered AID conditional daf-12 allele in daf-7(e1372) background with ubiquitous TIR1 expression. Reference: Aghayeva et al., submitted
OH14989 ieSi60 II; daf-7(e1372) III; daf-12(ot874[daf-12::TagRFP-T::AID]) X. ieSi60 [myo-2p::TIR1::mRuby + unc-119(+)] II. Pharyngeal muscle-specific depletion of DAF-12 in the presence of auxin. Reference: Aghayeva et al., submitted
OH15913 daf-7(e1372) III; daf-12(ot874[daf-12::TagRFP::AID]) X; otIs730. otIs730 [UPN::TIR1::mTur2 + inx-6(prom18)::TagRFP]. Temperature sensitive dauer constitutive. Maintain at 15C. UPN (Ultra Pan-Neuronal) promoter contains four short pan-neuronal promoters fused together (unc-11::rgef-1::ehs-1::ric-19). Panneuronal depletion of DAF-12 in the presence of auxin. Reference: Aghayeva et al., submitted
VC243 daf-12(ok493) X. F11A1.3, F11A1.2. Superficially wild type. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VT3922 lin-28(n719) I; daf-12(ma497[daf-12::gfp]) hbl-1(ma430[hbl-1::mScarlet-I]) X. Precocious heterochronic phenotypes as preciously reported for lin-28(n719). Endogenous daf-12 locus tagged with GFP. hbl-1(ma430) is a CRISPR/Cas9-edited allele, which contains a linker and the mScarlet-I sequence integrated in-frame with hbl-1. Reference: Ilbay O & Ambros V. Development. 2019 Nov 6;146(21).
VT3923 maIs105 V; daf-12(ma498[daf-12::mScarlet-I]) X. maIs105 [col-19p::GFP] V. mScarlet-I tag inserted at C-terminus of endogenous daf-12 locus through CRISPR/Cas9-engineering. Reference: Ilbay O & Ambros A. Development. 2019 Oct 9. pii: dev.183111. doi: 10.1242/dev.183111.